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anti-Smad2 antibody :: Rabbit Smad2 Polyclonal Antibody

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Catalog # MBS2536165
Unit / Price
  0.02 mL  /  $110 +1 FREE 8GB USB
  0.06 mL  /  $150 +1 FREE 8GB USB
  0.12 mL  /  $225 +1 FREE 8GB USB
  0.2 mL  /  $360 +1 FREE 8GB USB
Western Blot (WB)
Product Name

Smad2, Polyclonal Antibody

Popular Item
Also Known As

Smad2 Polyclonal Antibody

Product Synonym Names
SMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
467
OMIM
601366
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen Affinity Purified
Concentration
1 mg/ml (lot specific)
Immunogen
Synthesized peptide derived from human Smad2 around the non-phosphorylation site of S250.
Buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-101153
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Smad2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Smad2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes for anti-Smad2 antibody
WB: 1:500-1:2000, IHC: 1:50-1:200, ELISA: 1:5000-1:20000

Western Blot (WB) of anti-Smad2 antibody
Western Blot analysis of various cells with Smad2 Polyclonal Antibody.
anti-Smad2 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for Smad2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Related Accession #
Molecular Weight
Calculated MW: 52
Observed MW: 52
NCBI Official Full Name
Smad2
NCBI Official Synonym Full Names
SMAD family member 2
NCBI Official Symbol
SMAD2  [Similar Products]
NCBI Official Synonym Symbols
JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
  [Similar Products]
NCBI Protein Information
mothers against decapentaplegic homolog 2
UniProt Protein Name
Mothers against decapentaplegic homolog 2
UniProt Synonym Protein Names
JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2
UniProt Gene Name
SMAD2  [Similar Products]
UniProt Synonym Gene Names
MADH2; MADR2; MAD homolog 2; Mothers against DPP homolog 2; hMAD-2; SMAD 2; Smad2; hSMAD2  [Similar Products]
UniProt Entry Name
SMAD2_HUMAN
NCBI Summary for Smad2
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Comments for Smad2
SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding; double-stranded DNA binding; metal ion binding; phosphatase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; SMAD binding; transcription activator binding; transcription factor activity; transcription factor binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin protein ligase binding

Biological Process: activin receptor signaling pathway; anterior/posterior pattern formation; cell fate commitment; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; embryonic foregut morphogenesis; endoderm formation; gastrulation; gene expression; in utero embryonic development; insulin secretion; lung development; mesoderm formation; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; organ growth; palate development; pancreas development; paraxial mesoderm morphogenesis; pericardium development; positive regulation of BMP signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; post-embryonic development; primary microRNA processing; regulation of binding; regulation of transforming growth factor beta receptor signaling pathway; response to glucose stimulus; SMAD protein complex assembly; somatic stem cell maintenance; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; ureteric bud development; zygotic determination of dorsal/ventral axis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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