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anti-SMAD4 antibody :: Rabbit SMAD4 Polyclonal Antibody

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Catalog # MBS2542362
Unit / Price
Scan QR to view Datasheet
  0.02 mL  /  $110 +1 FREE 8GB USB
  0.06 mL  /  $150 +1 FREE 8GB USB
  0.12 mL  /  $225 +1 FREE 8GB USB
  0.2 mL  /  $360 +1 FREE 8GB USB
Product Name

SMAD4, Polyclonal Antibody

Popular Item
Full Product Name

SMAD4 Polyclonal Antibody

Product Synonym Names
JIP; DPC4; MADH4; MYHRS
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen Affinity Purification
Concentration
1.8mg/ml (lot specific)
Immunogen
Fusion protein of human SMAD4
Buffer
PBS with 0.05% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-1909 / sc-1908 / sc-7154
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-SMAD4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SMAD4 antibody
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Product Categories/Family for anti-SMAD4 antibody
Applications Tested/Suitable for anti-SMAD4 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SMAD4 antibody
ELISA: 1:1000-2000
IHC: 1:50-200
WB: 1:200-500

Immunohistochemistry (IHC) of anti-SMAD4 antibody
Immunohistochemistry of paraffin-embedded Human cervical cancer using SMAD4 Polyclonal Antibody at dilution of 1/40
anti-SMAD4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SMAD4 antibody
Immunohistochemistry of paraffin-embedded Human thyroid cancer using SMAD4 Polyclonal Antibody at dilution of 1/40
anti-SMAD4 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-SMAD4 antibody
Western Blot analysis of Human liver cancer tissue and Hela cells using SMAD4 Polyclonal Antibody at dilution of 1:150
anti-SMAD4 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SMAD4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
60 kDa
NCBI Official Full Name
SMAD4, partial
NCBI Official Synonym Full Names
SMAD family member 4
NCBI Official Symbol
SMAD4  [Similar Products]
NCBI Official Synonym Symbols
JIP; DPC4; MADH4; MYHRS
  [Similar Products]
NCBI Protein Information
mothers against decapentaplegic homolog 4
UniProt Protein Name
Mothers against decapentaplegic homolog 4
UniProt Synonym Protein Names
Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4
UniProt Gene Name
SMAD4  [Similar Products]
UniProt Synonym Gene Names
DPC4; MADH4; MAD homolog 4; Mothers against DPP homolog 4; SMAD 4; Smad4; hSMAD4  [Similar Products]
UniProt Entry Name
SMAD4_HUMAN
NCBI Summary for SMAD4
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq, Oct 2009]
UniProt Comments for SMAD4
SMAD4: transcription factor that mediates signal transduction by the transforming growth factor superfamily. The common smad (co-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. Promotes binding of the Smad2/Smad4/Fast-1 complex to DNA and provides an activation function required for Smad1 or Smad2 to stimulate transcription.

Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: DNA binding; identical protein binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity; transforming growth factor beta receptor, common-partner cytoplasmic mediator activity

Biological Process: BMP signaling pathway; cellular iron ion homeostasis; negative regulation of cell growth; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; palate development; positive regulation of BMP signaling pathway; positive regulation of histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; SMAD protein complex assembly; somatic stem cell maintenance; transforming growth factor beta receptor signaling pathway

Disease: Juvenile Polyposis Syndrome; Juvenile Polyposis/hereditary Hemorrhagic Telangiectasia Syndrome; Myhre Syndrome; Pancreatic Cancer
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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