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anti-SNAI2 antibody :: Rabbit SNAI2 Polyclonal Antibody

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Catalog # MBS126496
Unit / Price
  0.05 mL  /  $230 +1 FREE 8GB USB
  0.1 mL  /  $305 +1 FREE 8GB USB
  0.2 mL  /  $460 +1 FREE 8GB USB
Product Name

SNAI2, Polyclonal Antibody

Popular Item
Also Known As

SNAI2 Polyclonal Antibody

Product Synonym Names
SNAI2; MGC10182; SLUG; SLUGH1; WS2D
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
268
OMIM
gene 608890
3D Structure
ModBase 3D Structure for O43623
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human SNAI2
Calculated Molecular Weight
30kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-SNAI2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SNAI2 antibody
Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4,5). The tumor suppressor protein p53 induces Slug expression in gamma-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).
Product Categories/Family for anti-SNAI2 antibody
Applications Tested/Suitable for anti-SNAI2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-SNAI2 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-SNAI2 antibody
Western blot analysis of extracts of various cell lines, using SNAI2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
anti-SNAI2 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SNAI2 antibody
Immunohistochemistry of paraffin-embedded human liver injury using SNAI2 Antibody at dilution of 1:100 (40x lens).
anti-SNAI2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SNAI2 antibody
Immunohistochemistry of paraffin-embedded rat brain using SNAI2 Antibody at dilution of 1:100 (40x lens).
anti-SNAI2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SNAI2 antibody
Immunohistochemistry of paraffin-embedded rat spleen using SNAI2 Antibody at dilution of 1:100 (40x lens).
anti-SNAI2 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-SNAI2 antibody
Immunohistochemistry of paraffin-embedded human liver cancer using SNAI2 Antibody at dilution of 1:100 (40x lens).
anti-SNAI2 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-SNAI2 antibody
Immunofluorescence analysis of A549 cells using SNAI2 antibody. Blue: DAPI for nuclear staining.
anti-SNAI2 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for SNAI2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
268
NCBI Official Full Name
Zinc finger protein SNAI2
NCBI Official Synonym Full Names
snail family zinc finger 2
NCBI Official Symbol
SNAI2  [Similar Products]
NCBI Official Synonym Symbols
SLUG; WS2D; SLUGH1; SNAIL2
  [Similar Products]
NCBI Protein Information
zinc finger protein SNAI2; snail homolog 2; protein snail homolog 2; neural crest transcription factor SLUG; slug (chicken homolog), zinc finger protein
UniProt Protein Name
Zinc finger protein SNAI2
UniProt Synonym Protein Names
Neural crest transcription factor Slug; Protein snail homolog 2
Protein Family
UniProt Gene Name
SNAI2  [Similar Products]
UniProt Synonym Gene Names
SLUG; SLUGH  [Similar Products]
UniProt Entry Name
SNAI2_HUMAN
NCBI Summary for SNAI2
This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]
UniProt Comments for SNAI2
Snail2: Transcriptional repressor. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains). Expressed in placenta and adult heart, pancreas, liver, kidney and skeletal muscle. Belongs to the snail C2H2-type zinc-finger protein family.

Protein type: Apoptosis; Motility/polarity/chemotaxis; C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: cytoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; metal ion binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; substrate-bound cell migration, cell release from substrate; Notch signaling pathway; regulation of osteoblast differentiation; neural crest cell development; negative regulation of chondrocyte differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; osteoblast differentiation; negative regulation of DNA damage response, signal transduction by p53 class mediator; sensory perception of sound; pigmentation; white fat cell differentiation; epithelial to mesenchymal transition; positive regulation of fat cell differentiation; positive regulation of histone acetylation; regulation of chemokine production; negative regulation of cell adhesion mediated by integrin; positive regulation of cell migration

Disease: Waardenburg Syndrome, Type 2d; Piebald Trait
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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