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anti-SKIL antibody :: Rabbit SnoN Polyclonal Antibody

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Catalog # MBS151484
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

SnoN (SKIL), Polyclonal Antibody

Popular Item
Also Known As

SnoN Antibody

Product Synonym Names
SnoN; SNO; SnoA; SnoI; SnoN; SNO; Ski-like protein; Ski-related oncogene; SKI-like oncogene
Antibody/Peptide Pairs
SnoN peptide (MBS152549) is used for blocking the activity of SnoN antibody (MBS151484)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
684
OMIM
165340
3D Structure
ModBase 3D Structure for P12757
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
SnoN Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Conjugate
Unconjugated
Immunogen
SnoN antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human SnoN.
Buffer
SnoN Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
SnoN antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-SKIL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SKIL antibody
SnoN Antibody: TGF-beta is a ubiquitously expressed cytokine that signals through the Smad protein family to regulate numerous cellular processes such as embryonic development and tumorigenesis. This signaling is negatively regulated by Ski, the mammalian homolog of the transforming protein of an avian retrovirus that induces oncogenic transformation of chicken embryo cells, and the related protein SnoN. Like Ski, SnoN functions by binding to the Smad proteins and preventing their phosphorylation, thereby inhibiting their ability to bind DNA and activate the transcription of downstream genes. SnoN is located primarily in the nucleus in cancer tissues or cells, but in the cytoplasm in normal tissues or primary epithelial cells. There are at least four alternately spliced isoforms of SnoN; SnoN antibody will recognize all isoforms (SnoN, SnoN2, SnoI, and SnoA).
Applications Tested/Suitable for anti-SKIL antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF)
Application Notes for anti-SKIL antibody
SnoN antibody can be used for detection of SnoN by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-SKIL antibody
Western blot analysis of SnoN in A431 cell lysate with SnoN antibody at (A) 0.5 and (B) 1 μg/mL.
anti-SKIL antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-SKIL antibody
Immunohistochemistry of SnoN in mouse lung tissue with SnoN antibody at 5 μg/mL.
anti-SKIL antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-SKIL antibody
Immunofluorescence of SnoN in Mouse Lung cells with SnoN antibody at 20 μg/mL.
anti-SKIL antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for SKIL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
74,784 Da
NCBI Official Full Name
ski-like protein isoform 1
NCBI Official Synonym Full Names
SKI-like proto-oncogene
NCBI Official Symbol
NCBI Official Synonym Symbols
SNO; SnoA; SnoI; SnoN
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NCBI Protein Information
ski-like protein; SKI-like oncogene; ski-related oncogene snoN
UniProt Protein Name
Ski-like protein
UniProt Synonym Protein Names
Ski-related oncogene; Ski-related protein
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
SKIL_HUMAN
NCBI Summary for SKIL
The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Comments for SKIL
SKIL: May have regulatory role in cell division or differentiation in response to extracellular signals. Interacts with SMAD2, SMAD3 and RNF111. Isoform 1 interacts with WWP1. Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle. Belongs to the SKI family. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3q26

Cellular Component: nucleoplasm; PML body; protein complex; cytoplasm; acrosome; nucleus

Molecular Function: protein domain specific binding; protein binding; DNA binding; protein complex binding; chromatin binding; SMAD binding; transcription corepressor activity

Biological Process: transcription initiation from RNA polymerase II promoter; skeletal muscle development; transcription, DNA-dependent; blastocyst formation; negative regulation of transcription from RNA polymerase II promoter; response to antibiotic; regulation of apoptosis; negative regulation of cell differentiation; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; positive regulation of axonogenesis; gene expression; spermatogenesis; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle arrest; lymphocyte homeostasis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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