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anti-SHH antibody :: Rabbit Sonic Hedgehog Polyclonal Antibody

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Catalog # MBS175434
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Product Name

Sonic Hedgehog (SHH), Polyclonal Antibody

Also Known As

Anti-Sonic Hedgehog antibody

Product Synonym Names
Sonic hedgehog protein(SHH); sonic hedgehog; HHG 1 antibody; HHG-1 antibody; HHG1 antibody; HLP 3 antibody; HLP3 antibody; Holoprosencephaly 3 antibody; HPE 3 antibody; HPE3 antibody; MCOPCB5 antibody; SHH antibody; SHH_HUMAN antibody; SMMC I antibody; SMMCI antibody; Sonic Hedgehog(Drosophila) homolog antibody; sonic hedgehog homolog(Drosophila) antibody; Sonic hedgehog homolog antibody; Sonic hedgehog protein antibody; Sonic hedgehog protein C-product antibody; TPT antibody; TPTPS antibody
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
462
OMIM
gene 611638
3D Structure
ModBase 3D Structure for Q15465
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog (34-50aa RHPKKLTPLAYKQFIPN), identical to the related mouse and rat sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-SHH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-SHH antibody
Description: Rabbit IgG polyclonal antibody for Sonic hedgehog protein(SHH) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined; however, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.
Applications Tested/Suitable for anti-SHH antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin

Testing Data of anti-SHH antibody
anti-SHH antibody Testing Data image
Testing Data #2 of anti-SHH antibody
anti-SHH antibody Testing Data #2 image
NCBI/Uniprot data below describe general gene information for SHH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
49,607 Da
NCBI Official Full Name
Sonic hedgehog protein
NCBI Official Synonym Full Names
sonic hedgehog
NCBI Official Symbol
NCBI Official Synonym Symbols
TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
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NCBI Protein Information
sonic hedgehog protein; sonic hedgehog homolog
UniProt Protein Name
Sonic hedgehog protein
UniProt Synonym Protein Names
HHG-1Cleaved into the following 2 chains:Sonic hedgehog protein N-product; Sonic hedgehog protein C-product
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
SHH_HUMAN
NCBI Summary for SHH
This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]
UniProt Comments for SHH
SHH: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. Belongs to the hedgehog family.

Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Oncoprotein; Cell development/differentiation

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; plasma membrane; extracellular region; cytosol; nucleus; lipid raft

Molecular Function: peptidase activity; laminin-1 binding; morphogen activity; protein binding; glycosaminoglycan binding; zinc ion binding; patched binding; calcium ion binding; glycoprotein binding

Biological Process: prostate gland development; central nervous system development; positive regulation of transcription, DNA-dependent; embryonic skeletal development; telencephalon regionalization; embryonic foregut morphogenesis; male genitalia development; neural crest cell migration; inner ear development; hindbrain development; embryonic limb morphogenesis; positive regulation of neuroblast proliferation; camera-type eye development; neuron fate commitment; myotube differentiation; intermediate filament organization; positive regulation of skeletal muscle cell proliferation; osteoblast development; positive regulation of cell division; regulation of proteolysis; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of apoptosis; embryonic forelimb morphogenesis; axon guidance; granule cell precursor proliferation; ventral midline development; spinal cord dorsal/ventral patterning; palate development; positive regulation of striated muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; thalamus development; Bergmann glial cell differentiation; negative regulation of T cell proliferation; pancreas development; positive regulation of cell proliferation; forebrain development; thyroid gland development; heart looping; vasculogenesis; negative regulation of cell migration; positive thymic T cell selection; intein-mediated protein splicing; regulation of odontogenesis; androgen metabolic process; spinal cord motor neuron differentiation; pattern specification process; odontogenesis of dentine-containing teeth; regulation of cell proliferation; stem cell development; embryonic development; negative regulation of cell differentiation; positive regulation of protein import into nucleus; dorsal/ventral pattern formation; ureteric bud branching; hindgut morphogenesis; lung development; negative regulation of alpha-beta T cell differentiation; heart development; CD4-positive or CD8-positive, alpha-beta T cell lineage commitment; T cell differentiation in the thymus; lymphoid progenitor cell differentiation; Wnt receptor signaling pathway through beta-catenin; proteolysis; embryonic pattern specification; positive regulation of T cell differentiation in the thymus; cell-cell signaling; embryonic digestive tract morphogenesis; midbrain development; ectoderm development; positive regulation of smoothened signaling pathway; positive regulation of oligodendrocyte differentiation; oligodendrocyte development; activation of hh target transcription factor; striated muscle development; endocytosis; positive regulation of skeletal muscle development; negative thymic T cell selection; patterning of blood vessels; branching morphogenesis of a tube; polarity specification of anterior/posterior axis; metanephros development; cell fate specification; embryonic hindlimb morphogenesis; positive regulation of Wnt receptor signaling pathway; dorsoventral neural tube patterning; smoothened signaling pathway; organ formation; hair follicle morphogenesis; thymus development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; positive regulation of immature T cell proliferation in the thymus; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; formation of anatomical boundary; myoblast differentiation; limb bud formation; establishment of cell polarity; neuroblast proliferation; blood coagulation; cell development; positive regulation of alpha-beta T cell differentiation

Disease: Solitary Median Maxillary Central Incisor; Schizencephaly; Microphthalmia, Isolated, With Coloboma 5; Holoprosencephaly 3
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