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anti-STIM1 antibody :: Rabbit STIM1 Polyclonal Antibody

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Catalog # MBS150530
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

STIM1, Polyclonal Antibody

Full Product Name

STIM1 Antibody

Product Synonym Names
STIM1; GOK; TAM; IMD10; D11S4896E; GOK; Stromal interaction molecule 1; stromal interaction molecule 1
Antibody/Peptide Pairs
STIM1 peptide (MBS152297) is used for blocking the activity of STIM1 antibody (MBS150530)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
phenotype 615887
3D Structure
ModBase 3D Structure for Q13586
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
At least two isoforms of STIM1 are known to exist; this antibody will detect only the larger form. This STIM1 antibody is predicted to have no cross-reactivity to STIM2.
Purity/Purification
STIM1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.
Buffer
STIM1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
STIM1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-STIM1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-STIM1 antibody
STIM1 Antibody: In T lymphocytes, the sole pathway for Ca2+ entry following antigen-receptor binding is through store-operated Ca2+-release-activated Ca2+ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca2+ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca2+ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca2+ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca2+ entry.
Applications Tested/Suitable for anti-STIM1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-STIM1 antibody
STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL.

Western Blot (WB) of anti-STIM1 antibody
Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 μg/mL.
anti-STIM1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-STIM1 antibody
Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 μg/mL.
anti-STIM1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for STIM1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Predicted: 59; 75; 87 kDa

Observed: 88 kDa
NCBI Official Full Name
stromal interaction molecule 1 isoform 2
NCBI Official Synonym Full Names
stromal interaction molecule 1
NCBI Official Symbol
STIM1  [Similar Products]
NCBI Official Synonym Symbols
GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
  [Similar Products]
NCBI Protein Information
stromal interaction molecule 1
UniProt Protein Name
Stromal interaction molecule 1
UniProt Gene Name
STIM1  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
STIM1_HUMAN
NCBI Summary for STIM1
This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
UniProt Comments for STIM1
STIM1: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Ubiquitously expressed in various human primary cells and tumor cell lines.

Protein type: Membrane protein, integral; Endoplasmic reticulum; Calcium-binding

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: microtubule; endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: identical protein binding; microtubule plus-end binding; protein binding; calcium ion binding

Biological Process: activation of store-operated calcium channel activity; calcium ion transport; detection of calcium ion; regulation of calcium ion transport; blood coagulation

Disease: Myopathy, Tubular Aggregate, 1; Immunodeficiency 10
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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