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anti-STK4 antibody :: Rabbit STK4 Polyclonal Antibody

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Catalog # MBS9127929
Unit / Price
  0.05 mL  /  $230 +1 FREE 8GB USB
  0.1 mL  /  $305 +1 FREE 8GB USB
  0.2 mL  /  $460 +1 FREE 8GB USB
Product Name

STK4, Polyclonal Antibody

Popular Item
Also Known As

STK4 Polyclonal Antibody

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
462
OMIM
604965
3D Structure
ModBase 3D Structure for Q13043
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human STK4
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-STK4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-STK4 antibody
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Applications Tested/Suitable for anti-STK4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes for anti-STK4 antibody
WB 1:500 - 1:2000
IHC 1:20 - 1:200
IF 1:20 - 1:100
IP 1:20 - 1:50

Western Blot (WB) of anti-STK4 antibody
Western blot analysis of extracts of various cell lines, using STK4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.
anti-STK4 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-STK4 antibody
Immunohistochemistry of paraffin-embedded human stomach using STK4 antibody at dilution of 1:100 (40x lens).
anti-STK4 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-STK4 antibody
Immunohistochemistry of paraffin-embedded human esophagus using STK4 antibody at dilution of 1:100 (40x lens).
anti-STK4 antibody Immunohistochemistry (IHC) (IHC) image
Immunoprecipitation (IP) of anti-STK4 antibody
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug STK4 antibody. Western blot was performed from the immunoprecipitate using STK4 antibody at a dilition of 1:1000.
anti-STK4 antibody Immunoprecipitation (IP) (IP) image
NCBI/Uniprot data below describe general gene information for STK4. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56kDa
NCBI Official Full Name
serine/threonine-protein kinase 4
NCBI Official Synonym Full Names
serine/threonine kinase 4
NCBI Official Symbol
NCBI Official Synonym Symbols
KRS2; MST1; YSK3; TIIAC
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase 4
UniProt Protein Name
Serine/threonine-protein kinase 4
UniProt Synonym Protein Names
Mammalian STE20-like protein kinase 1; MST-1
UniProt Gene Name
UniProt Synonym Gene Names
KRS2; MST1; MST-1; MST1/N; MST1/C  [Similar Products]
UniProt Entry Name
STK4_HUMAN
NCBI Summary for STK4
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]
UniProt Comments for STK4
MST1: a protein kinase of the STE20 family. Proteolytically activated by caspase during apoptosis. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Phosphorylation at serine 327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage.

Protein type: Autophagy; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; Protein kinase, STE; STE group; STE20 family; MST subfamily

Chromosomal Location of Human Ortholog: 20q11.2-q13.2

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein dimerization activity; identical protein binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; magnesium ion binding; transcription factor binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: central nervous system development; positive regulation of protein binding; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; cell morphogenesis; negative regulation of organ growth; signal transduction; protein amino acid phosphorylation; patterning of blood vessels; keratinocyte differentiation; negative regulation of cell proliferation; peptidyl-serine phosphorylation; positive regulation of fat cell differentiation; neural tube formation

Disease: T-cell Immunodeficiency, Recurrent Infections, And Autoimmunity With Or Without Cardiac Malformations
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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