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anti-STRAD antibody :: Rabbit STRAD Polyclonal Antibody

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Catalog # MBS9600427
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

STRAD, Polyclonal Antibody

Popular Item
Also Known As

STRAD Antibody

Product Synonym Names
Protein kinase LYK5; Serologically defined breast cancer antigen NY BR 96; STE20 related adapter protein; STRAD alpha; STRAD
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
394
3D Structure
ModBase 3D Structure for Q7RTN6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
STRAD antibody detects endogenous levels of STRAD
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human STRAD
Subcellular Location
Cytoplasmic and Nuclear
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-STRAD antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-STRAD antibody
Description: STRAD a pseudokinase which, in complex with CAB39, binds to and activates LKB1. Relocates LKB1 from the nucleus to the cytoplasm. Plays an essential role in LKB1-mediated G1 cell cycle arrest. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Function: Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Subunit Structure: Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.
Similarity: The protein kinase domain is predicted to be catalytically inactive. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Applications Tested/Suitable for anti-STRAD antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-STRAD antibody
WB: 1:500-1:2000
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-STRAD antibody
Western blot analysis on HepG2 cell lysate using STRAD Antibody, The lane on the left is treated with the antigen-specific peptide.
anti-STRAD antibody Western Blot (WB) (WB) image
Immunofluorescene (IF) of anti-STRAD antibody
MBS9600427 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-STRAD antibody Immunofluorescene (IF) image
NCBI/Uniprot data below describe general gene information for STRAD. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 46 kDa
Predicted: 49 kDa
NCBI Official Full Name
STE20-related kinase adapter protein alpha isoform 2
NCBI Official Synonym Full Names
STE20-related kinase adaptor alpha
NCBI Official Symbol
STRADA  [Similar Products]
NCBI Official Synonym Symbols
LYK5; PMSE; Stlk; STRAD; NY-BR-96; STRAD alpha
  [Similar Products]
NCBI Protein Information
STE20-related kinase adapter protein alpha
UniProt Protein Name
STE20-related kinase adapter protein alpha
UniProt Synonym Protein Names
STE20-related adapter protein; Serologically defined breast cancer antigen NY-BR-96
UniProt Gene Name
STRADA  [Similar Products]
UniProt Synonym Gene Names
; STRAD alpha  [Similar Products]
NCBI Summary for STRAD
The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009]
UniProt Comments for STRAD
Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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Products associated withanti-STRAD antibody
 Reference Product  PubMed Publications
 STK11 antibody  >1 publications with STRAD and STK11
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