• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-TBC1D4-S588 antibody :: Rabbit TBC1D4-S588 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS9135643 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.05 mL  /  $235 +1 FREE 8GB USB
  0.1 mL  /  $350 +1 FREE 8GB USB
  0.2 mL  /  $525 +1 FREE 8GB USB
Western Blot (WB)
Product Name

TBC1D4-S588, Polyclonal Antibody

Popular Item
Full Product Name

Phospho-TBC1D4-S588 pAb

Product Synonym Names
AS160; NIDDM5
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
LGSVD
OMIM
FM207106 mRNA
3D Structure
ModBase 3D Structure for O60343
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Immunogen
A phospho specific peptide corresponding to residues surrounding S588 of human TBC1D4
Immunogen Species
Human
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Cytoplasm
Positive Samples
HeLa
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Other Notes
Small volumes of anti-TBC1D4-S588 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TBC1D4-S588 antibody
This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms.
Applications Tested/Suitable for anti-TBC1D4-S588 antibody
Western Blot (WB)
Application Notes for anti-TBC1D4-S588 antibody
WB: 1:500 - 1:2000

Western Blot (WB) of anti-TBC1D4-S588 antibody
Western blot analysis of extracts of HeLa cells, using Phospho-TBC1D4-S588 antibody at 1:2000 dilution. HeLa cells were treated by IGF-1 (50ng/ml) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 30s.
anti-TBC1D4-S588 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TBC1D4-S588. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Calculated: 53kDa; 60kDa; 139kDa; 145kDa; 146kDa
Observed: 160kDa
NCBI Official Full Name
TBC1 domain family member 4 isoform 2
NCBI Official Synonym Full Names
TBC1 domain family member 4
NCBI Official Symbol
TBC1D4  [Similar Products]
NCBI Official Synonym Symbols
AS160; NIDDM5
  [Similar Products]
NCBI Protein Information
TBC1 domain family member 4
UniProt Protein Name
TBC1 domain family member 4
UniProt Synonym Protein Names
Akt substrate of 160 kDa; AS160
UniProt Gene Name
TBC1D4  [Similar Products]
UniProt Synonym Gene Names
AS160; KIAA0603; AS160  [Similar Products]
NCBI Summary for TBC1D4-S588
This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
UniProt Comments for TBC1D4-S588
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000