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anti-TCIRG1 antibody :: Rabbit TCIRG1 Polyclonal Antibody

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Catalog # MBS150138
Unit / Price
  0.1 mg  /  $375 +1 FREE 8GB USB
Western Blot (WB)
Product Name

TCIRG1, Polyclonal Antibody

Also Known As

TCIRG1 Antibody

Product Synonym Names
TCIRG1; a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa; V-type proton ATPase 116 kDa subunit a isoform 3; Osteoclastic proton pump 116 kDa subunit; V-ATPase 116 kDa isoform a3; T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
Antibody/Peptide Pairs
TCIRG1 peptide (MBS152543) is used for blocking the activity of TCIRG1 antibody (MBS150138)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
830
OMIM
phenotype 604592
3D Structure
ModBase 3D Structure for Q13488
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform.
Purity/Purification
TCIRG1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
TCIRG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCIRG1.
Buffer
TCIRG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TCIRG1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-TCIRG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TCIRG1 antibody
TCIRG1 Antibody: The T-cell immune regulator 1 (TCIRG1) is the a3 subunit of the vacuolar-type proton transporting ATPase (V-ATPase), a proton pump in late endosomes and lysosomes that functions in the luminal acidification of these organelles. Genetic defects in the gene encoding this protein a responsible for a severe form of autosomal recessive osteopetrosis, a condition that leads to increased bone density, decreased bone strength, and inflammation in bone tissues. TCIRG1 is also required for the normal secretion of insulin and the bacteria-killing function of macrophages.
Applications Tested/Suitable for anti-TCIRG1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes for anti-TCIRG1 antibody
TCIRG1 antibody can be used for detection of TCIRG1 by Western blot at 0.5 mug/mL.

Western Blot (WB) of anti-TCIRG1 antibody
Western blot analysis of TCIRG1 in EL4 cell lysate with TCIRG1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
anti-TCIRG1 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TCIRG1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
91 kDa
NCBI Official Full Name
V-type proton ATPase 116 kDa subunit a isoform 3 isoform a
NCBI Official Synonym Full Names
T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
NCBI Official Symbol
TCIRG1  [Similar Products]
NCBI Official Synonym Symbols
a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa
  [Similar Products]
NCBI Protein Information
V-type proton ATPase 116 kDa subunit a isoform 3; OC-116 kDa; V-ATPase 116-kDa; T-cell immune response cDNA 7; ATPase, H+ transporting, 116kD; osteoclastic proton pump 116 kDa subunit; specific 116-kDa vacuolar proton pump subunit; vacuolar proton translocating ATPase 116 kDa subunit A; T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein a
UniProt Protein Name
V-type proton ATPase 116 kDa subunit a isoform 3
UniProt Synonym Protein Names
Osteoclastic proton pump 116 kDa subunit; OC-116 kDa; OC116; T-cell immune regulator 1; T-cell immune response cDNA7 protein; TIRC7; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3
Protein Family
UniProt Gene Name
TCIRG1  [Similar Products]
UniProt Synonym Gene Names
ATP6N1C; ATP6V0A3; V-ATPase 116 kDa isoform a3; OC-116 kDa; OC116; TIRC7  [Similar Products]
UniProt Entry Name
VPP3_HUMAN
NCBI Summary for TCIRG1
Through alternate splicing, this gene encodes two proteins with similarity to subunits of the vacuolar ATPase (V-ATPase) but the encoded proteins seem to have different functions. V-ATPase is a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, Jul 2008]
UniProt Comments for TCIRG1
TCIRG1: Part of the proton channel of V-ATPases. Seems to be directly involved in T-cell activation. Defects in TCIRG1 are the cause of osteopetrosis autosomal recessive type 1 (OPTB1); also called autosomal recessive Albers-Schonberg disease or infantile malignant osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. The features of OPTB1 are macrocephaly, progressive deafness and blindness, hepatosplenomegaly, and severe anemia beginning in early infancy or in fetal life. Deafness and blindness are generally thought to represent effects of pressure on nerves. Belongs to the V-ATPase 116 kDa subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, iron; Energy Metabolism - oxidative phosphorylation; Mitochondrial; Transporter; Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: phagocytic vesicle membrane; mitochondrion; integral to plasma membrane; lysosomal membrane; apical plasma membrane; plasma membrane; endosome membrane; vacuolar proton-transporting V-type ATPase complex

Molecular Function: transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism; ATPase binding

Biological Process: interaction with host; vacuolar acidification; proton transport; ATP synthesis coupled proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; positive regulation of cell proliferation; insulin receptor signaling pathway; cellular defense response; transferrin transport; transmembrane transport

Disease: Osteopetrosis, Autosomal Recessive 1
Research Articles on TCIRG1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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