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anti-TIMP3 antibody :: Rabbit TIMP3 Polyclonal Antibody

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Catalog # MBS126907
Unit / Price
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  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $275 +1 FREE 8GB USB
  0.2 mL  /  $430 +1 FREE 8GB USB
Product Name

TIMP3, Polyclonal Antibody

Popular Item
Full Product Name

TIMP3 Polyclonal Antibody

Product Synonym Names
HSMRK222; K222; K222TA2; SFD; TIMP3
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
phenotype 188826
3D Structure
ModBase 3D Structure for P35625
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Affinity Purification
Concentration
1mg/ml (lot specific)
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human TIMP3
Calculated Molecular Weight
24kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Other Notes
Small volumes of anti-TIMP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TIMP3 antibody
esultant inhibition of proteinase function (1,2). In addition to MMP inhibition, TIMPs have also been shown to interact with various membrane receptors on the cell surface. Some of these interactions include: TIMP1 with CD63, TIMP2 with alpha3beta1 integrin, and TIMP3 with VEGFR2, all of which result in distinct cellular effects (3). TIMPs are involved in a wide variety of biological functions, such as tumor angiogenesis and progression (4,5), wound healing, and vascular remodeling (6,7). Mutations in TIMP3 are associated with Sorsby's fundus dystrophy (8,9).
Product Categories/Family for anti-TIMP3 antibody
Applications Tested/Suitable for anti-TIMP3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-TIMP3 antibody
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200

Western Blot (WB) of anti-TIMP3 antibody
Western blot analysis of extracts of various cell lines, using TIMP3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
anti-TIMP3 antibody Western Blot (WB) (WB) image
Immunofluorescence (IF) of anti-TIMP3 antibody
Immunofluorescence analysis of MCF-7 cells using TIMP3 antibody. Blue: DAPI for nuclear staining.
anti-TIMP3 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for TIMP3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
211
NCBI Official Full Name
Metalloproteinase inhibitor 3
NCBI Official Synonym Full Names
TIMP metallopeptidase inhibitor 3
NCBI Official Symbol
TIMP3  [Similar Products]
NCBI Official Synonym Symbols
SFD; K222; K222TA2; HSMRK222
  [Similar Products]
NCBI Protein Information
metalloproteinase inhibitor 3; TIMP-3; MIG-5 protein; protein MIG-5; tissue inhibitor of metalloproteinases 3
UniProt Protein Name
Metalloproteinase inhibitor 3
UniProt Synonym Protein Names
Protein MIG-5; Tissue inhibitor of metalloproteinases 3; TIMP-3
UniProt Gene Name
TIMP3  [Similar Products]
UniProt Synonym Gene Names
TIMP-3  [Similar Products]
UniProt Entry Name
TIMP3_HUMAN
NCBI Summary for TIMP3
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]
UniProt Comments for TIMP3
TIMP3: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. Interacts with EFEMP1. Belongs to the protease inhibitor I35 (TIMP) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; basement membrane; nucleus

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding

Biological Process: negative regulation of metalloenzyme activity; visual perception; response to cytokine stimulus; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis

Disease: Fundus Dystrophy, Pseudoinflammatory, Of Sorsby
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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