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anti-TPPP antibody :: Goat TPPP/p25 Polyclonal Antibody

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Catalog # MBS422281
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

TPPP/p25, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-TPPP/p25 Antibody

Product Synonym Names
brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, p24, p25, p25alpha, TPPP/p25, TPPP1, tubulin polymerization promoting protein, tubulin polymerization-promoting protein, TPPP
Product Synonym Gene Name
Antibody/Peptide Pairs
TPPP/p25 peptide (MBS427274) is used for blocking the activity of TPPP/p25 antibody (MBS422281)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
DPSKDRAAKR LSLES
OMIM
608773
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse, Rat

Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-DPSKDRAAKRLSLES, from the internal region of the protein sequence according to NP_008961.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-TPPP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-TPPP antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-TPPP antibody
Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26kDa band observed in Human Brain (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 23.7kDa according to NP_008961.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Acevedo et al, Exp Cell Res. 2007 Dec 10;313(20):4091-106; PMID: 18028908). Recommended concentration: 0.001-0.01ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Cerebellum shows strong staining of axonal bundles through the granular layer. Recommended concentration: 1-2ug/ml. Paraffin embedded Human Cortex. Recommended concentration: 3.75ug/ml.

Western Blot (WB) of anti-TPPP antibody
(0. 001ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-TPPP antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-TPPP antibody
(0. 001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
anti-TPPP antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-TPPP antibody
(2ug/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
anti-TPPP antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry of anti-TPPP antibody
Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.
anti-TPPP antibody Immunohistochemistry (IHC) image
Immunohistochemistry of anti-TPPP antibody
(3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-TPPP antibody Immunohistochemistry (IHC) image
NCBI/Uniprot data below describe general gene information for TPPP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Related Accession #
Molecular Weight
23,694 Da
NCBI Official Full Name
tubulin polymerization-promoting protein
NCBI Official Synonym Full Names
tubulin polymerization promoting protein
NCBI Official Symbol
NCBI Official Synonym Symbols
p24; p25; TPPP1; TPPP/p25; p25alpha
  [Similar Products]
NCBI Protein Information
tubulin polymerization-promoting protein
UniProt Protein Name
Tubulin polymerization-promoting protein
UniProt Synonym Protein Names
25 kDa brain-specific protein; TPPP/p25; p24; p25-alpha
UniProt Gene Name
UniProt Synonym Gene Names
TPPP1; TPPP  [Similar Products]
UniProt Entry Name
TPPP_HUMAN
UniProt Comments for TPPP
TPPP: May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown. Belongs to the TPPP family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: cytoplasm; microtubule; myelin sheath; nucleus; perinuclear region of cytoplasm

Molecular Function: microtubule binding; protein binding; tubulin binding

Biological Process: microtubule bundle formation; microtubule polymerization; positive regulation of protein complex assembly; positive regulation of protein polymerization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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