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anti-TH antibody :: Rabbit Tyrosine Hydroxylase Polyclonal Antibody

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Catalog # MBS9600886
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

Tyrosine Hydroxylase (TH), Polyclonal Antibody

Popular Item
Full Product Name

Phospho-Tyrosine Hydroxylase (Ser40) Antibody

Product Synonym Names
Dystonia 14; DYT14; DYT5b; EC 1.14.16.2; OTTHUMP00000011225; OTTHUMP00000011226; ple; Protein Pale; TH; The; TY3H_HUMAN; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyrosine 3-hydroxylase; Tyrosine 3-monooxygenase; Tyrosine hydroxylase
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P07101
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Phospho-Tyrosine Hydroxylase (Ser40) antibody detects endogenous levels of Tyrosine Hydroxylase only when phosphorylated at Serine 40
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human Tyrosine Hydroxylase around the phosphorylation site of Serine 40
Tissue Specificity
Mainly expressed in the brain and adrenal glands.
Conjugation
Unconjugated
Epitope
Phospho Ser40
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-TH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-TH antibody
Description: Tyrosine hydroxylase (EC 1.14.16.2) is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
Function: Plays an important role in the physiology of adrenergic neurons.
Similarity: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Applications Tested/Suitable for anti-TH antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-TH antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Western Blot (WB) of anti-TH antibody
Western blot analysis of Tyrosine Hydroxylase phosphorylation expression in RAW264.7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
anti-TH antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-TH antibody
MBS9600886 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-TH antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-TH antibody
MBS9600886 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-TH antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-TH antibody
MBS9600886 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-TH antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-TH antibody
Western blot analysis of Phospho-Tyrosine Hydroxylase (Ser40) expression in various lysates
anti-TH antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 60 kDa
Predicted: 59 kDa
NCBI Official Full Name
tyrosine 3-monooxygenase isoform b
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
NCBI Official Synonym Symbols
TYH; DYT14; DYT5b
  [Similar Products]
NCBI Protein Information
tyrosine 3-monooxygenase
UniProt Protein Name
Tyrosine 3-monooxygenase
UniProt Synonym Protein Names
Tyrosine 3-hydroxylase; TH
UniProt Gene Name
UniProt Synonym Gene Names
TYH; TH  [Similar Products]
NCBI Summary for TH
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for TH
Plays an important role in the physiology of adrenergic neurons.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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