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anti-UBC9 antibody :: Rabbit Ubc9 Polyclonal Antibody

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Catalog # MBS616215
Unit / Price
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  0.1 mg  /  $620 +1 FREE 8GB USB
anti-UBC9 antibody
Product Name

Ubc9, Polyclonal Antibody

Full Product Name

Ubc9 (Ubiquitin-Conjugating Enzyme 9)

Product Synonym Names
Anti -Ubc9 (Ubiquitin-Conjugating Enzyme 9)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 4; NC_003075.7 (13915820..13917420, complement)
3D Structure
ModBase 3D Structure for P35132
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
Reacts with human, mouse, rat and golden hamster ubiquitin-conjugating enzyme 9 (Ubc9) protein. On Western blots, it identifies the target band at ~18kD. A band at ~13kD is observed in mouse spleen homogenates. Reactivity has been confirmed HEK293, HepG2, HL-60 cell lysates and mouse spleen homogenates.
Purity/Purification
Affinity Purified
Epitope-affinity purified from rabbit antiserum.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Immunogen
Synthetic peptide derived from an internal region of the human, mouse, rat and golden hamster Ubc9 proteins.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-UBC9 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-UBC9 antibody
Ubiquitin-conjugating enzyme 9 (Ubc9)1-3 is a component of the ubiquitin-mediated proteolytic SUMO (sentrin) pathway that targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization.4,5 Ubc9 primarily mediates SUMOlation but not ubiquitinylation.6,7 An E3 ubiquitin ligase systematically transfers Ub and Ublike molecules from E2 conjugating enzymes to the targeted substrate.8 Ubc9, like other E2 family members, contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins, and the E2 member, and it shares a conserved Ubc domain of approximately 16kD.9 More than 30 different vertebrate proteins are potential candidates for SUMOlation by Ubc9.10 Many Ubc9-interacting proteins contain a PEST sequence, a stretch of at least 12 amino acids rich in P, E, D, S, and T amino acid residues.10-13 Substrates for Ubc9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, indicating that Ubc9 may regulate various cellular processes including cell cycle progression and differentiation.14,15 Many Ubc9-interacting proteins have been implicated in the maintenance of vertebrate genome integrity, including the tumor suppressor protein p53,16 the promyelocytic leukemia protein (Pml),17 the poly(ADP-ribose) polymerase PARP18 and the homologous recombination proteins Rad51/52.13,16,19 Ubc9 is predominantly present in the nucleus, and at the nuclear pore complex.10, 20-22 Ubc9's distribution across the nuclear membrane, nuclear versus cytoplasmic, is important in controlling the subcellular localization of SUMOs and/or SUMOlated proteins, the assembly of Pml bodies, and the homologous recombination process mediated by Rad51.13
Product Categories/Family for anti-UBC9 antibody
Applications Tested/Suitable for anti-UBC9 antibody
Western Blot (WB)
Application Notes for anti-UBC9 antibody
Suitable for use in Western Blot.
Dilution: Western Blotting: 1-3ug/ml
NCBI/Uniprot data below describe general gene information for UBC9. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
16,553 Da[Similar Products]
NCBI Official Full Name
SUMO-conjugating enzyme UBC9
NCBI Official Symbol
NCBI Official Synonym Symbols
T13J8.70; T13J8_70; UBC9; ubiquitin conjugating enzyme 9; UBIQUITIN-PROTEIN LIGASE
  [Similar Products]
NCBI Protein Information
SUMO-conjugating enzyme UBC9
UniProt Protein Name
SUMO-conjugating enzyme UBC9
UniProt Synonym Protein Names
UBCAT4B; Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2 9; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase 9
UniProt Gene Name
UniProt Synonym Gene Names
UBC4B  [Similar Products]
UniProt Entry Name
UBC9_ARATH
NCBI Summary for UBC9
ubiquitin conjugating enzyme
UniProt Comments for UBC9
Function: Accepts the ubiquitin-like protein SUMO/SMT3 from the E1 complex and catalyzes its covalent attachment to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.

Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathway: Protein modification; protein sumoylation.

Subunit structure: Interacts with CHIP. Ref.8

Tissue specificity: Highest expression in young stems and old leaves. Lowest levels in floral buds, anthers and young leaves. Ref.3

Developmental stage: Up-regulated during senescence, but not during the G0 to S phase transition. Ref.2

Induction: Not induced by heat shock or wounding. Ref.2

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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