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anti-UNG antibody :: Rabbit UNG1 Polyclonal Antibody

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Catalog # MBS150242
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

UNG1, Polyclonal Antibody

Full Product Name

UNG1 Antibody

Product Synonym Names
UNG1; DGU; UDG; UNG1; UNG2; HIGM4; HIGM5; UNG15; DGU; Uracil-DNA glycosylase; uracil-DNA glycosylase
Product Gene Name
Antibody/Peptide Pairs
UNG1 peptide (MBS153076) is used for blocking the activity of UNG1 antibody (MBS150242)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
gene 608106
3D Structure
ModBase 3D Structure for P13051
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
UNG1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
UNG1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human UNG1.
Buffer
UNG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
UNG1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-UNG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-UNG antibody
UNG1 Antibody: The human uracil-DNA glycosylase (UNG) gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms through differentially regulated promotes and alternative splicing. While UNG2 is the major enzyme in the base excision repair pathway that removes uracil residues from nuclear DNA that arise through either misincorporation during replication or cytosine deamination, inhibition of UNG1 by uracil glycosylase inhibitor did not lead to increased levels of spontaneous or induced mitochondrial DNA mutations. However, decreased levels of UNG activity and increased oxidative damage to mitochondrial DNA were seen in older mice, suggesting that mitochondrial DNA repair mechanisms may be involved in various neurodegenerative disorders in an age-dependent manner. This UNG1 antibody will not cross-react with UNG2.
Applications Tested/Suitable for anti-UNG antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-UNG antibody
UNG1 antibody can be used for the detection of UNG1 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-UNG antibody
Western blot analysis of UNG1 in C2C12 cell lysate with UNG1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
anti-UNG antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-UNG antibody
Immunohistochemistry of UNG1 in human heart tissue with UNG1 antibody at 2 μg/mL.
anti-UNG antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-UNG antibody
Immunofluorescence of UNG1 in Human Heart cells with UNG1 antibody at 20 μg/mL.
anti-UNG antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for UNG. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
33,924 Da
NCBI Official Full Name
uracil-DNA glycosylase isoform UNG1
NCBI Official Synonym Full Names
uracil-DNA glycosylase
NCBI Official Symbol
NCBI Official Synonym Symbols
DGU; UDG; UNG1; UNG2; HIGM4; HIGM5; UNG15
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NCBI Protein Information
uracil-DNA glycosylase; UDG; UNG; uracil-DNA glycosylase 1, uracil-DNA glycosylase 2
UniProt Protein Name
Uracil-DNA glycosylase
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
UNG_HUMAN
NCBI Summary for UNG
This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010]
UniProt Comments for UNG
UNG: Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Defects in UNG are a cause of immunodeficiency with hyper-IgM type 5 (HIGM5). A rare immunodeficiency syndrome characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE. It results in a profound susceptibility to bacterial infections. Belongs to the uracil-DNA glycosylase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.2.27; Hydrolase

Chromosomal Location of Human Ortholog: 12q23-q24.1

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; uracil DNA N-glycosylase activity

Biological Process: viral reproduction; base-excision repair, AP site formation; base-excision repair; depyrimidination; somatic hypermutation of immunoglobulin genes; somatic recombination of immunoglobulin gene segments; DNA repair; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 5
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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