• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-USP33 antibody :: Rabbit USP33 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS9603725
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

USP33, Polyclonal Antibody

Popular Item
Also Known As

USP33 Antibody

Product Synonym Names
Deubiquitinating enzyme 33; EC 3 1 2 15; hVDU1; KIAA1097; MGC16868; OTTHUMP00000011234; OTTHUMP00000011330; OTTHUMP00000011331; pVHL interacting deubiquitinating enzyme 1; Ubiquitin carboxyl terminal hydrolase 33; Ubiquitin carboxyl-terminal hydrolase 33; Ubiquitin specific peptidase 33; Ubiquitin specific processing protease 33; ubiquitin specific protease 33; Ubiquitin thioesterase 33; Ubiquitin thiolesterase 33; Ubiquitin-specific-processing protease 33; UBP33_HUMAN; USP 33; Usp33; VDU 1; VDU1; VHL interacting deubiquitinating enzyme 1; VHL-interacting deubiquitinating enzyme 1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
942
3D Structure
ModBase 3D Structure for Q8TEY7
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
USP33 antibody detects endogenous levels of USP33.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human USP33.
Subcellular Location
Cytoplasm > Perinuclear Region. According to PubMed:12865408, it localizes in the Endoplasmic Reticulum; however the relevance of such result is unclear.
Tissue Specificity
Widely expressed.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-USP33 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-USP33 antibody
Function: Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.
Subunit Structure: Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with ARRB1, ARRB2, ADRB2, DIO2 and ROBO1. Interacts with SELENBP1; in a selenium-dependent manner. Interacts with CCP110.
Post-translational Modifications: Ubiquitinated via a VHL-dependent pathway for proteasomal degradation.
Similarity: The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 86 is replaced by a Glu residue. Belongs to the peptidase C19 family. USP20/USP33 subfamily.
Applications Tested/Suitable for anti-USP33 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-USP33 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200

Immunohistochemistry (IHC) of anti-USP33 antibody
MBS9603725 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-USP33 antibody Immunohistochemistry (IHC) (IHC) image
Western Blot (WB) of anti-USP33 antibody
Western blot analysis of extracts of mouse brain tissue, using USP33 antibody.
anti-USP33 antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for USP33. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 103 kDa; 107 kDa
Predicted: 107 kDa
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 33 isoform 1
NCBI Official Synonym Full Names
ubiquitin specific peptidase 33
NCBI Official Symbol
USP33  [Similar Products]
NCBI Official Synonym Symbols
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 33
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 33
UniProt Synonym Protein Names
Deubiquitinating enzyme 33; Ubiquitin thioesterase 33; Ubiquitin-specific-processing protease 33; VHL-interacting deubiquitinating enzyme 1; hVDU1
UniProt Gene Name
USP33  [Similar Products]
UniProt Synonym Gene Names
KIAA1097; VDU1; hVDU1  [Similar Products]
NCBI Summary for USP33
This gene encodes a deubiquinating enzyme important in a variety of processes, including Slit-dependent cell migration and beta-2 adrenergic receptor signaling. The protein is negatively regulated through ubiquitination by von Hippel-Lindau tumor protein (VHL). Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]
UniProt Comments for USP33
Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000