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anti-VPS4A antibody :: Rabbit VPS4A Polyclonal Antibody

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Catalog # MBS7045153
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

VPS4A, Polyclonal Antibody

Popular Item
Also Known As

VPS4A Antibody

Product Synonym Names
Vacuolar protein sorting-associated protein 4A; Protein SKD2; VPS4-1; hVPS4; VPS4A
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
437
OMIM
609982
3D Structure
ModBase 3D Structure for Q9UN37
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Species
Human
Immunogen
Recombinant human Vacuolar protein sorting-associated protein 4A protein (N-120AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21461 / sc-21463 / sc-32922
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-VPS4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-VPS4A antibody
Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission (PubMed:24814515). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).
Applications Tested/Suitable for anti-VPS4A antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-VPS4A antibody
Western blot
All lanes:  VPS4Aantibody at 4.28ug/ml
Lane 1 : Mouse kidney tissue
Lane 2 : Rat gonadal tissue
Lane 3 : Mouse brain tissue
Lane 4 : U87 whole cell lysate
Lane 5 : MCF7 whole cell lysate
Secondary
Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa

anti-VPS4A antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-VPS4A antibody
Immunohistochemistry of paraffin-embedded human lung tissue using MBS7045153 at dilution 1:100
anti-VPS4A antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-VPS4A antibody
Immunohistochemistry of paraffin-embedded human testis tissue using MBS7045153 at dilution 1:100
anti-VPS4A antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for VPS4A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
48,898 Da
NCBI Official Full Name
vacuolar protein sorting-associated protein 4A
NCBI Official Synonym Full Names
vacuolar protein sorting 4 homolog A
NCBI Official Symbol
VPS4A  [Similar Products]
NCBI Official Synonym Symbols
SKD1; SKD2; VPS4; SKD1A; VPS4-1
  [Similar Products]
NCBI Protein Information
vacuolar protein sorting-associated protein 4A
UniProt Protein Name
Vacuolar protein sorting-associated protein 4A
UniProt Synonym Protein Names
Protein SKD2; VPS4-1; hVPS4
UniProt Gene Name
VPS4A  [Similar Products]
UniProt Synonym Gene Names
hVPS4  [Similar Products]
UniProt Entry Name
VPS4A_HUMAN
NCBI Summary for VPS4A
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq, Jul 2008]
UniProt Comments for VPS4A
VPS4A: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Involved in cytokinesis. Belongs to the AAA ATPase family.

Protein type: EC 3.6.4.6; Vesicle

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: centrosome; cytoplasm; cytosol; early endosome; endosome; late endosome; lysosome; midbody; nucleus; perinuclear region of cytoplasm; plasma membrane; spindle pole; vacuolar membrane

Molecular Function: ATP binding; ATPase activity; microtubule-severing ATPase activity; protein binding; protein C-terminus binding; protein domain specific binding

Biological Process: abscission; cell division; cell separation during cytokinesis; cytoplasmic microtubule organization and biogenesis; endosomal vesicle fusion; endosome transport; intracellular cholesterol transport; membrane budding; mitotic metaphase plate congression; negative regulation of cytokinesis; nuclear organization and biogenesis; regulation of protein localization; release of virus from host; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; vacuole organization and biogenesis; vesicle-mediated transport; viral infectious cycle
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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