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anti-WASF2 antibody :: Rabbit WAVE2 (Central region) Polyclonal Antibody

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Catalog # MBS472070
Unit / Price
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  0.1 mL  /  $280 +1 FREE 8GB USB
Testing Data
Product Name

WAVE2 (Central region) (WASF2), Polyclonal Antibody

Full Product Name

WAVE2 (Central region)

Product Synonym Names
Wiskott-Aldrich syndrome verproline-homologous protein 2; Scar2; WASF2
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 1; NC_000001.10 (27732126..27816669, complement). Location: 1p36.11
3D Structure
ModBase 3D Structure for Q05BU7
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Specificity
This antibody was affinity purified using WAVE2 (Central region) peptide (without carrier). The antibody detects a 78 kDa* protein corresponding to the molecular mass of WAVE2 on SDS-PAGE immunoblots of rat PC12 cells.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Immunogen
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the central region of human WAVE2. This sequence has high homology with similar regions in rat and mouse WAVE2, and has low homology to similar regions in WAVE1 and WAVE3.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
Other Notes
Small volumes of anti-WASF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-WASF2 antibody
The Wiskott-Aldrich syndrome protein (WASP) family is involved in various pathways that regulate actin cytoskeletal organization. This family includes WASP, N-WASP, and three WAVE/SCAR isoforms, WAVEs 1, 2, and 3. WAVE proteins play key roles in actin-mediated cell events, such as membrane ruffling and lamellipodia formation. WAVEs contain an Nterminal WAVE homology domain, a basic domain, a Proline-rich region, and carboxy terminal verprolin, cofilin, and acidic (VCA) region. WAVEs are thought to act downstream of the Rac GTPase, connecting Rac activation to induction of Arp 2/3-mediated actin polymerization. Regulation of WAVE activity can occur through tyrosine phosphorylation. Src phosphorylation of WAVE1 at Tyr-125 enhances binding to the Arp2/3 complex, and is required for WAVE inhibition of Arp2/3-mediated stress fiber formation. By contrast, WAVE2 phosphorylation of Tyr-150 by Abl may enhance Arp2/3 complex actin nucleation and microspike formation in fibroblasts. Thus, site-specific tyrosine phosphorylation may be important for controlling specific activities of WAVE proteins.
Applications Tested/Suitable for anti-WASF2 antibody
ELISA, Western Blot
Application Notes for anti-WASF2 antibody
ELISA: 1:2000
Western Blot: 1:1000

Testing Data of anti-WASF2 antibody
anti-WASF2 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for WASF2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
21,410 Da[Similar Products]
NCBI Official Full Name
wiskott-Aldrich syndrome protein family member 2 isoform 2
NCBI Official Synonym Full Names
WAS protein family, member 2
NCBI Official Symbol
WASF2  [Similar Products]
NCBI Official Synonym Symbols
SCAR2; WAVE2; dJ393P12.2
  [Similar Products]
NCBI Protein Information
wiskott-Aldrich syndrome protein family member 2; IMD2; protein WAVE-2; OTTHUMP00000003471; WASP family protein member 2; WASP family Verprolin-homologous protein 2; verprolin homology domain-containing protein 2; suppressor of cyclic-AMP receptor (WASP-family)
UniProt Protein Name
WASF2 protein
UniProt Gene Name
WASF2  [Similar Products]
UniProt Entry Name
Q05BU7_HUMAN
NCBI Summary for WASF2
This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
UniProt Comments for WASF2
WAVE2: Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. Directly interacts with BRK1. Expressed in all tissues with strongest expression in placenta, lung, and peripheral blood leukocytes, but not in skeletal muscle. Belongs to the SCAR/WAVE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: ruffle; lamellipodium; early endosome; intracellular; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: protein binding; protein complex binding; actin binding

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; lamellipodium biogenesis; negative regulation of stress fiber formation; actin filament-based movement; ameboidal cell migration; innate immune response; Rac protein signal transduction; angiogenesis; actin cytoskeleton organization and biogenesis; vascular endothelial growth factor receptor signaling pathway
Product References and Citations for anti-WASF2 antibody
• Ardern, H. et al. (2006) Cell Motil. Cytosk. 63:6.
• Leng, Y. et al. (2005) PNAS 102(4):1098.
• Suetsugu, S. et al. (1999) Bioch. Biophs. Res. Comm. 260:296.
• Miki, H. et al. (1999) J Biol. Chem. 274(39):27605.

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Disclaimer
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