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anti-WTAP antibody :: Rabbit WTAP Polyclonal Antibody

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Catalog # MBS7050204
Unit / Price
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  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

WTAP, Polyclonal Antibody

Popular Item
Full Product Name

WTAP Antibody

Product Synonym Names
Pre-mRNA-splicing regulator WTAP; Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein; WTAP; KIAA0105
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF374416 mRNA
3D Structure
ModBase 3D Structure for Q15007
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant Human Pre-mRNA-splicing regulator WTAP protein (1-151AA)
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Conjugation
Non-conjugated
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-55438 / sc-55439 / sc-55440 / sc-55441 / sc-67389
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-WTAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-WTAP antibody
Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed: 24316715, PubMed: 24407421, PubMed: 24981863). Acts as a mRNA splicing regulator (PubMed: 12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed: 17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed: 17095724).
Applications Tested/Suitable for anti-WTAP antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-WTAP antibody
WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200

Western Blot (WB) of anti-WTAP antibody
Western Blot
Positive WB detected in: Mouse kidney tissue,Rat heart tissue,Rat liver tissue
All lanes: WTAP antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size:45,18 KDa
Observed band size: 45 KDa

anti-WTAP antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-WTAP antibody
Immunohistochemistry of paraffin-embedded human testis tissue using MBS7050204 at dilution of 1:100
anti-WTAP antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-WTAP antibody
Immunofluorescent analysis of Hela cells using MBS7050204 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-WTAP antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for WTAP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,801 Da
NCBI Official Full Name
pre-mRNA-splicing regulator WTAP isoform 1
NCBI Official Synonym Full Names
Wilms tumor 1 associated protein
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
pre-mRNA-splicing regulator WTAP
UniProt Protein Name
Pre-mRNA-splicing regulator WTAP
UniProt Synonym Protein Names
Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein
UniProt Gene Name
UniProt Synonym Gene Names
KIAA0105; hFL(2)D  [Similar Products]
UniProt Entry Name
FL2D_HUMAN
NCBI Summary for WTAP
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012]
UniProt Comments for WTAP
WTAP: Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. Belongs to the fl(2)d family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Spliceosome

Chromosomal Location of Human Ortholog: 6q25-q27

Cellular Component: nuclear membrane; nuclear speck; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: gene expression; regulation of alternative nuclear mRNA splicing, via spliceosome
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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