• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-YAP antibody :: Rabbit YAP Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS9413065
Unit / Price
Scan QR to view Datasheet
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Western Blot (WB)
Product Name

YAP, Polyclonal Antibody

Full Product Name

YAP (Phospho-Ser127) Antibody

Product Synonym Names
YAP65; YAP1
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
120433
3D Structure
ModBase 3D Structure for P46937
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
The antibody detects endogenous level of YAP only when phosphorylated at serine 127.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of serine127(A-H-S(p)-S-P) derived from Human YAP.
Modification
Phospho-Ser127
Target Name
YAP
Preparation and Storage
Store at -20 degree C/1 year
Other Notes
Small volumes of anti-YAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-YAP antibody
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact.Sorrentino G, et al. (2014) Nat Cell Biol 16, 357-66 Yang S, et al. (2014) Cell Signal 26, 343-51 Sun W, et al. (2014) Mol Biol Rep 41, 947-56
Product Categories/Family for anti-YAP antibody
Applications Tested/Suitable for anti-YAP antibody
Western Blot (WB)
Application Notes for anti-YAP antibody
Western blotting: 1:500~1:1000

Western Blot (WB) of anti-YAP antibody
Western blot analysis of extracts from COS7 tissue using YAP (Phospho-Ser127) antibody.The lane on the right is treated with the antigen-specific peptide.
anti-YAP antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for YAP. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54,942 Da
NCBI Official Full Name
yorkie homolog isoform 1
NCBI Official Synonym Full Names
Yes-associated protein 1
NCBI Official Symbol
NCBI Official Synonym Symbols
YAP; YKI; COB1; YAP2; YAP65
  [Similar Products]
NCBI Protein Information
transcriptional coactivator YAP1
UniProt Protein Name
Transcriptional coactivator YAP1
UniProt Synonym Protein Names
Protein yorkie homolog; Yes-associated protein YAP65 homolog
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
YAP65; Yes-associated protein 1  [Similar Products]
UniProt Entry Name
YAP1_HUMAN
NCBI Summary for YAP
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
UniProt Comments for YAP
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000