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anti-ZNF148 antibody :: Rabbit ZNF148 Polyclonal Antibody

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Catalog # MBS9600566
Unit / Price
  0.1 mL  /  $255 +1 FREE 8GB USB
  0.2 mL  /  $295 +1 FREE 8GB USB
Product Name

ZNF148, Polyclonal Antibody

Popular Item
Full Product Name

ZNF148 Antibody

Product Synonym Names
Transcription factor ZBP-89; Transcription factor ZBP89; ZBP89; Zinc finger DNA binding protein 89; Zinc finger DNA-binding protein 89; Zinc finger protein 148; ZN148_HUMAN; ZNF 148; ZNF148
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q9UQR1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Specificity
ZNF148 antibody detects endogenous levels of ZNF148
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (lot specific)
Immunogen
A synthesized peptide derived from human ZNF148
Subcellular Location
Nucleus.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Other Notes
Small volumes of anti-ZNF148 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ZNF148 antibody
Description: ZNF148 a transcription factor. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. Belongs to the krueppel C2H2-type zinc-finger protein family.
Function: Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
Subunit Structure: Interacts with HNRNPDL (PubMed:15190078). Interacts with the 5FMC complex; the interaction requires association with CHTOP. Interacts with CAVIN1 (By similarity).
Post-translational Modifications: Sumoylated with SUMO2. Desumoylated by SENP3, resulting in the stimulation of transcription of its target genes (By similarity).
Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family.
Applications Tested/Suitable for anti-ZNF148 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ZNF148 antibody
WB: 1:500-1:2000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-ZNF148 antibody
MBS9600566 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-ZNF148 antibody Immunofluorescence (IF) image
Western Blot (WB) of anti-ZNF148 antibody
Western blot analysis on HepG2 cell lysate using ZNF148 Antibody. The lane on the left is treated with the antigen-specific peptide.
anti-ZNF148 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ZNF148 antibody
Western blot analysis of extracts from various samples, using ZNF148 Antibody. Lane 1: rat brain treated with blocking peptide. Lane 2: rat brain; Lane 3: mouse brain;
anti-ZNF148 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ZNF148 antibody
MBS9600566 at 1/100 staining human Kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-ZNF148 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ZNF148. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
Observed: 89 kDa
Predicted: 89 kDa
NCBI Official Full Name
zinc finger protein 148 isoform a
NCBI Official Synonym Full Names
zinc finger protein 148
NCBI Official Symbol
ZNF148  [Similar Products]
NCBI Official Synonym Symbols
BERF-1; BFCOL1; GDACCF; ZBP-89; ZFP148; pHZ-52; HT-BETA
  [Similar Products]
NCBI Protein Information
zinc finger protein 148
UniProt Protein Name
Zinc finger protein 148
UniProt Synonym Protein Names
Transcription factor ZBP-89; Zinc finger DNA-binding protein 89
Protein Family
UniProt Gene Name
ZNF148  [Similar Products]
UniProt Synonym Gene Names
ZBP89  [Similar Products]
NCBI Summary for ZNF148
The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies. [provided by RefSeq, Feb 2017]
UniProt Comments for ZNF148
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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