• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-AUP1 antibody :: Rabbit anti-Human, Pig Ancient Ubiquitous Protein 1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS2032556 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $340 +1 FREE 8GB USB
  1 mg  /  $800 +1 FREE 8GB USB
Product Name

Ancient Ubiquitous Protein 1 (AUP1), Polyclonal Antibody

Popular Item
Full Product Name

Ancient Ubiquitous Protein 1 (AUP1) Polyclonal Antibody

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
AF100754 mRNA
3D Structure
ModBase 3D Structure for Q9Y679
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Pig
Specificity
The antibody is a rabbit polyclonal antibody raised against AUP1. It has been selected for its ability to recognize AUP1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Human
Fragment
AUP1 (Val201~Asp410)
Quality Control
Content: The quality control contains recombinant AUP1 (Val201~Asp410) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2064753
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-AUP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-AUP1 antibody
Applications Tested/Suitable for anti-AUP1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-AUP1 antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-AUP1 antibody
Western Blot: Sample: Human Serum; Primary Ab: 1ug/ml Rabbit Anti-Human AUP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-AUP1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-AUP1 antibody
Western Blot: Sample: Recombinant protein.
anti-AUP1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AUP1 antibody
DAB staining on fromalin fixed paraffin- embedded Kidney tissue)
anti-AUP1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-AUP1 antibody
DAB staining on IHC-P; Samples: Human Stomach Tissue)
anti-AUP1 antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-AUP1 antibody
Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: AUP1 knockout Jurkat cell lysate; Predicted MW: 53,46,41kd Observed MW: 45kd Primary Ab: 1ug/ml Rabbit Anti-Human AUP1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-AUP1 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for AUP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
41,385 Da
NCBI Official Full Name
ancient ubiquitous protein 1
NCBI Official Synonym Full Names
AUP1, lipid droplet regulating VLDL assembly factor
NCBI Official Symbol
NCBI Protein Information
ancient ubiquitous protein 1
UniProt Protein Name
Ancient ubiquitous protein 1
UniProt Gene Name
NCBI Summary for AUP1
The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
UniProt Comments for AUP1
AUP1: May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. Belongs to the AUP1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: extracellular exosome; Hrd1p ubiquitin ligase ERAD-L complex; integral component of endoplasmic reticulum membrane; membrane

Molecular Function: protein binding; ubiquitin binding

Biological Process: ER-associated protein catabolic process; regulation of catalytic activity; retrograde protein transport, ER to cytosol
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000