• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-FUM antibody :: Rabbit anti-Human, Pig Fumarase Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS2032542 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $170 +1 FREE 8GB USB
  0.1 mg  /  $220 +1 FREE 8GB USB
  0.2 mg  /  $320 +1 FREE 8GB USB
  1 mg  /  $760 +1 FREE 8GB USB
Product Name

Fumarase, Polyclonal Antibody

Popular Item
Full Product Name

Fumarase (FUM) Polyclonal Antibody

Product Synonym Names
FH; Fumarate Hydratase, mitochondrial
Product Gene Name
Matching Pairs
Unconjugated Antibody: Fumarase (MBS2032542)
Immunogen: Fumarase (MBS2033222)
Matching Pairs
Unconjugated Antibody: Fumarase (MBS2032542)
APC-CY7 Conjugated Antibody: Fumarase (FUM) (MBS2060690)
Matching Pairs
Unconjugated Antibody: Fumarase (MBS2032542)
PE Conjugated Antibody: Fumarase (FUM) (MBS2060691)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
U59309 mRNA
3D Structure
ModBase 3D Structure for P07954
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Pig
Specificity
The antibody is a rabbit polyclonal antibody raised against FUM. It has been selected for its ability to recognize FUM in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Human
Fragment
FUM (Ala45~Asn188)
Quality Control
Content: The quality control contains recombinant FUM (Ala45~Asn188) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2060692
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-FUM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-FUM antibody
Applications Tested/Suitable for anti-FUM antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-FUM antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-FUM antibody
Western Blot: Sample: Lane1: Human Serum; Lane2: Human Hela Cells; Lane3: Porcine Liver Tissue.
anti-FUM antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-FUM antibody
Western Blot: Sample: Recombinant FUM, Human.
anti-FUM antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-FUM antibody
DAB staining on IHC-P; Samples: Human Stomach Tissue.
anti-FUM antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-FUM antibody
DAB staining on IHC-P; Samples: Human Liver Tissue
anti-FUM antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-FUM antibody
DAB staining on IHC-P; Samples: Human Kidney Tissue
anti-FUM antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-FUM antibody
Knockout Validation: Lane 1: Wild-type Hela cell lysate; Lane 2: FUM knockout Hela cell lysate; Predicted MW: 55,50kd Observed MW: 50kd Primary Ab: 1ug/ml Rabbit Anti-Human FUM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-FUM antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for FUM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
50,213 Da
NCBI Official Full Name
fumarate hydratase, mitochondrial
NCBI Official Synonym Full Names
fumarate hydratase
NCBI Official Symbol
NCBI Official Synonym Symbols
MCL; FMRD; LRCC; HLRCC; MCUL1
  [Similar Products]
NCBI Protein Information
fumarate hydratase, mitochondrial
UniProt Protein Name
Fumarate hydratase, mitochondrial
UniProt Gene Name
UniProt Synonym Gene Names
Fumarase  [Similar Products]
NCBI Summary for FUM
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
UniProt Comments for FUM
fumarase: a metabolic enzyme that participates in the tricarboxylic acid cycle that catalyzes the conversion of (S)-malate into fumarate + H2O. There are two substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors. Fumarate accumulates in the cell when FH is inactivated. Fumarate inhibits the dioxygenases that hydroxylate the transcription factor HIF and leads to its degradation by VHL. Since HIF turns on oncogenic pathways, FH has apparent tumor suppressor activity. Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC), a highly metastatic form of RCC. Defects in FH are the cause of fumarase deficiency (FD) also known as fumaricaciduria. FD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia. Cells derived from a patient with HLRCC exhibit compromised oxidative phosphorylation, dependence on anaerobic glycolysis, rapid glycolytic flux, and overexpression of lactate dehydrogenase A (LDHA) and GLUT1. Two human isoforms are produced by alternative initiation. The longer isoform is mitochondrial, while the shorter form, missing residues 1-43, is cytoplasmic.

Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.2.1.2; Lyase; Mitochondrial; Tumor suppressor

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: cytoplasm; extracellular exosome; mitochondrial matrix; mitochondrion; tricarboxylic acid cycle enzyme complex

Molecular Function: fumarate hydratase activity; protein binding

Biological Process: fumarate metabolic process; homeostasis of number of cells within a tissue; malate metabolic process; tricarboxylic acid cycle

Disease: Fumarase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000