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anti-GPX1 antibody :: Goat Glutathione Peroxidase 1 Polyclonal Antibody

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Catalog # MBS421332
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

Glutathione Peroxidase 1 (GPX1), Polyclonal Antibody

Popular Item
Also Known As

Goat anti-Glutathione Peroxidase 1 (isoform1) Antibody

Product Synonym Names
glutathione peroxidase 1; HGNC: 4553; GPX1; GSHPX1; MGC14399; MGC88245; glutathione peroxidase 1 antibody; HGNC: 4553 antibody; GPX1 antibody; GSHPX1 antibody; MGC14399 antibody; MGC88245 antibody; Glutathione Peroxidase 1; Glutathione Peroxidase 1 (isoform1)
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
203
Immunogen Sequence
REALPAPSDD ATA
OMIM
138320
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Pig; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-REALPAPSDDATA, from the internal region of the protein sequence according to NP_000572.2.
Epitope
Internal region
Note
This antibody is expected to recognise isoform 1 (NP_000572.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-GPX1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GPX1 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-GPX1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration, 3-5ug/ml. Frozen sections of Porcin Kidney shows staining of cytoplasm in PCT epithelial cells. Recommended concentration, 20ug/ml.
Western Blot: Approx 22kDa band observed in Human Liver and Kidney lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-GPX1 antibody
(1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-GPX1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-GPX1 antibody
(3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-GPX1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-GPX1 antibody
(20ug/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia
anti-GPX1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for GPX1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
10,289 Da
NCBI Official Full Name
glutathione peroxidase 1 isoform 1
NCBI Official Synonym Full Names
glutathione peroxidase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
GPXD; GSHPX1
  [Similar Products]
NCBI Protein Information
glutathione peroxidase 1
UniProt Protein Name
Glutathione peroxidase 1
UniProt Synonym Protein Names
Cellular glutathione peroxidase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GPx-1; GSHPx-1  [Similar Products]
UniProt Entry Name
GPX1_HUMAN
NCBI Summary for GPX1
This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for GPX1
GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.

Protein type: EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: cytoplasm; cytosol; mitochondrial matrix; mitochondrion

Molecular Function: glutathione peroxidase activity; SH3 domain binding

Biological Process: angiogenesis involved in wound healing; blood vessel endothelial cell migration; cell redox homeostasis; endothelial cell development; fat cell differentiation; glutathione metabolic process; heart contraction; hydrogen peroxide catabolic process; induction of apoptosis by oxidative stress; interaction with symbiont; lipoxygenase pathway; myoblast proliferation; negative regulation of caspase activity; negative regulation of inflammatory response to antigenic stimulus; positive regulation of protein kinase B signaling cascade; protein amino acid oxidation; purine nucleotide catabolic process; regulation of gene expression, epigenetic; regulation of mammary gland epithelial cell proliferation; regulation of neuron apoptosis; response to gamma radiation; response to hydrogen peroxide; response to hydroperoxide; response to reactive oxygen species; response to selenium ion; response to symbiotic bacterium; response to xenobiotic stimulus; sensory perception of sound; skeletal muscle fiber development; skeletal muscle regeneration; thermoregulation; triacylglycerol metabolic process; UV protection; vasodilation

Disease: Glutathione Peroxidase Deficiency
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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