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anti-SH2D1A antibody :: Goat SH2D1A/SLAM associated protein Polyclonal Antibody

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Catalog # MBS421463
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Product Name

SH2D1A/SLAM associated protein, Polyclonal Antibody

Full Product Name

Goat anti-SH2D1A/SLAM associated protein Antibody

Product Synonym Names
Goat Anti-SH2D1A / SAP Antibody; SH2D1A; SH2 domain protein 1A, Duncan's disease (lymphoproliferative syndrome); HGNC: 10820; DSHP; EBVS; IMD5; LYP; MTCP1; SAP; XLP; XLPD; SH2 domain protein 1A; SLAM (signaling lymphocyte activation molecule) associated protein; T cell signal transduction; SH2D1A antibody; SH2 domain protein 1A; Duncan\1s disease (lymphoproliferative syndrome) antibody; HGNC: 10820 antibody; DSHP antibody; EBVS antibody; IMD5 antibody; LYP antibody; MTCP1 antibody; SAP antibody; XLP antibody; XLPD antibody; SH2 domain protein 1A antibody; SLAM (signaling lymphocyte activation molecule) associated protein antibody; T cell signal transduction molecule SAP antibody; RP5-1052M9.3 antibody; FLJ18687 antibody; FLJ92177 antibody; Duncan's disease (lymphoproliferative syndrome) antibody; Duncan's disease antibody; SLAM-associated protein antibody; T cell signal transduction molecule SAP; signaling lymphocyte activation molecule-associated protein antibody; SH2D1A/SLAM associated protein
Product Synonym Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
QYPVEKKSSA RSTQ
OMIM
300490
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Pig,
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QYPVEKKSSARSTQ, from the internal region of the protein sequence according to NP_002342.1; NP_001108409.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-SH2D1A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-SH2D1A antibody
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes for anti-SH2D1A antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 16kDa band observed in Human Lymph Node lysates (calculated MW of 14.2kDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx.17kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.2-0.6ug/ml.
Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from anonymous customer).

Western Blot (WB) of anti-SH2D1A antibody
(0.2ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-SH2D1A antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-SH2D1A antibody
HEK293 overexpressing SH2D1A (RC204723) and probed (mock transfection in first lane), tested by Origene.
anti-SH2D1A antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for SH2D1A. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2008 Certified Laboratory.NP_001108409.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
6,631 Da
NCBI Official Full Name
SH2 domain-containing protein 1A isoform 1
NCBI Official Synonym Full Names
SH2 domain containing 1A
NCBI Official Symbol
SH2D1A  [Similar Products]
NCBI Official Synonym Symbols
LYP; SAP; XLP; DSHP; EBVS; IMD5; XLPD; MTCP1; XLPD1; SAP/SH2D1A
  [Similar Products]
NCBI Protein Information
SH2 domain-containing protein 1A
UniProt Protein Name
SH2 domain-containing protein 1A
UniProt Synonym Protein Names
Duncan disease SH2-protein; Signaling lymphocytic activation molecule-associated protein; SLAM-associated protein; T-cell signal transduction molecule SAP
UniProt Gene Name
SH2D1A  [Similar Products]
UniProt Synonym Gene Names
DSHP; SAP; SLAM-associated protein  [Similar Products]
UniProt Entry Name
SH21A_HUMAN
NCBI Summary for SH2D1A
This gene encodes a protein that plays a major role in the bidirectional stimulation of T and B cells. This protein contains an SH2 domain and a short tail. It associates with the signaling lymphocyte-activation molecule, thereby acting as an inhibitor of this transmembrane protein by blocking the recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to its docking site. This protein can also bind to other related surface molecules that are expressed on activated T, B and NK cells, thereby modifying signal transduction pathways in these cells. Mutations in this gene cause lymphoproliferative syndrome X-linked type 1 or Duncan disease, a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus, with symptoms including severe mononucleosis and malignant lymphoma. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for SH2D1A
SH2D1A: Inhibitor of the SLAM self-association. Acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. Mediates interaction between FYN and SLAMF1. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3. Interacts with NTRK1, NTRK2 and NTRK3. Interacts with CD84, CD244, LY9, SLAMF1 and FYN. Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: Xq25

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity

Biological Process: adaptive immune response; cell-cell signaling; cellular defense response; humoral immune response; innate immune response; positive regulation of natural killer cell mediated cytotoxicity; positive regulation of signal transduction; regulation of immune response

Disease: Lymphoproliferative Syndrome, X-linked, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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