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anti-S17aH antibody :: Rabbit anti-Human, Rat 17-Alpha-Hydroxylase Polyclonal Antibody

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Catalog # MBS2032782 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $115 +1 FREE 8GB USB
  0.02 mg  /  $135 +1 FREE 8GB USB
  0.05 mg  /  $190 +1 FREE 8GB USB
  0.1 mg  /  $245 +1 FREE 8GB USB
  0.2 mg  /  $365 +1 FREE 8GB USB
  1 mg  /  $870 +1 FREE 8GB USB
Product Name

17-Alpha-Hydroxylase (S17aH), Polyclonal Antibody

Full Product Name

17-Alpha-Hydroxylase (S17aH) Polyclonal Antibody

Product Synonym Names
CYP17A1; CPT7; P450C17; 17-alpha-hydroxyprogesterone aldolase/17, 20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450, Family 17, Subfamily A, Polypeptide 1
Matching Pairs
Matching Pairs
Unconjugated Antibody: 17-Alpha-Hydroxylase (MBS2032782)
APC-CY7 Conjugated Antibody: 17-Alpha-Hydroxylase (S17aH) (MBS2062107)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P11715
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Specificity
The antibody is a rabbit polyclonal antibody raised against S17aH. It has been selected for its ability to recognize S17aH in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Organism Species
Rat
Fragment
S17aH (Thr201~Thr507)
Quality Control
Content: The quality control contains recombinant S17aH (Thr201~Thr507) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2062109
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-S17aH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-S17aH antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-S17aH antibody
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-S17aH antibody
Western Blot: Sample: Lane1: Rat Testis Tissue; Lane2: Human A549 Cells.
anti-S17aH antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-S17aH antibody
Western Blot: Sample: Recombinant S17aH, Rat.
anti-S17aH antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-S17aH antibody
DAB staining on IHC-P; Samples: Rat Testis Tissue
anti-S17aH antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-S17aH antibody
DAB staining on IHC-P; Samples: Rat Adrenal Gland Tissue.
anti-S17aH antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for S17aH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
57,250 Da
NCBI Official Full Name
steroid 17-alpha-hydroxylase/17,20 lyase
NCBI Official Synonym Full Names
cytochrome P450, family 17, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp17a1  [Similar Products]
NCBI Official Synonym Symbols
Cyp17
  [Similar Products]
NCBI Protein Information
steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Synonym Protein Names
17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17
UniProt Gene Name
Cyp17a1  [Similar Products]
UniProt Synonym Gene Names
Cyp17; Cytochrome P450c17  [Similar Products]
NCBI Summary for S17aH
steroid hydroxylase that comprises a main component of the steroidogenic pathway; activity culminates in corticosteriod and androgen production in adrenal gland and sex steroid production in gonads [RGD, Feb 2006]
UniProt Comments for S17aH
CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: EC 1.14.99.9; EC 4.1.2.30; Lipid Metabolism - C21-steroid hormone; Oxidoreductase

Chromosomal Location of Human Ortholog: 1q54

Cellular Component: axon; cell projection; cell soma; endoplasmic reticulum; intracellular membrane-bound organelle; membrane; mitochondrion

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; heme binding; iron ion binding; steroid 17-alpha-monooxygenase activity

Biological Process: adrenal gland development; androgen biosynthetic process; biphenyl metabolic process; dibenzo-p-dioxin metabolic process; glucocorticoid biosynthetic process; hippocampus development; hormone biosynthetic process; Leydig cell differentiation; male gonad development; organic acid metabolic process; ovulation; phenol-containing compound metabolic process; phthalate metabolic process; progesterone metabolic process; response to acetate; response to cAMP; response to cytokine; response to drug; response to herbicide; response to insecticide; response to ionizing radiation; response to methylmercury; response to nutrient levels; response to organic cyclic compound; response to organic substance; response to retinoic acid; response to steroid hormone; response to toxin; steroid biosynthetic process; steroid metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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