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anti-GHRL antibody :: Goat Ghrelin preproprotein Polyclonal Antibody

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Catalog # MBS421239
Unit / Price
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  0.1 mg  /  $300 +1 FREE 8GB USB
Immunohistochemistry (IHC)
Product Name

Ghrelin preproprotein (GHRL), Polyclonal Antibody

Full Product Name

Goat anti-Ghrelin preproprotein Antibody

Product Synonym Names
GHRL; MTLRP; ghrelin; ghrelin precursor; motilin-related peptide; preproghrelin; ghrelin/obestatin prepropeptide; obestatin; OTTHUMP00000207794; OTTHUMP00000207795; OTTHUMP00000207796; OTTHUMP00000207798; ghrelin, growth hormone secretagogue receptor liga; GHRL antibody; MTLRP antibody; ghrelin antibody; ghrelin precursor antibody; motilin-related peptide antibody; preproghrelin antibody; ghrelin/obestatin prepropeptide antibody; obestatin antibody; ghrelin/obestatin preprohormone antibody; OTTHUMP00000207794; antibody; OTTHUMP00000207795 antibody; OTTHUMP00000207796 antibody; OTTHUMP00000207798 antibody; ghrelin; growth hormone secretagogue receptor ligand antibody; ghrelin/obestatin antibody; Ghrelin preproprotein; Ghrelin preproprotein
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
QDILWEEAKE APADK
OMIM
605353
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Rat; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-QDILWEEAKEAPADK, from the C Terminus of the protein sequence according to NP_057446.1; NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1.
Epitope
C Terminus
Note
This antibody is expected to recognise all reported isoforms.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10368
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-GHRL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-GHRL antibody
Peptide ELISA (EIA), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for anti-GHRL antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Immunohistochemistry: In paraffin embedded Human Placenta shows staining of trophoblast cytoplasm. Recommended concentration, 2-5ug/ml.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the calculated size of this preproprotein of approx.12.9kDa according to NP_057446.1 and of its derivatives. Therefore we cannot recommend an optimal concentration and the product is investigative grade.
Immunocytochemsitry: This antibody has been successfully used in ICC for rat neurons as described in the following paper: Stoyanova et al, Regul Pept.2009 Nov 27;158(1-3): 86-90, PMID: 19540881.

Immunohistochemistry (IHC) of anti-GHRL antibody
(2ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-GHRL antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for GHRL. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001128413.1; NP_001128416.1; NP_001128417.1; NP_001128418.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
9,972 Da
NCBI Official Full Name
appetite-regulating hormone isoform 1 preproprotein
NCBI Official Synonym Full Names
ghrelin/obestatin prepropeptide
NCBI Official Symbol
NCBI Official Synonym Symbols
MTLRP
  [Similar Products]
NCBI Protein Information
appetite-regulating hormone
UniProt Protein Name
Appetite-regulating hormone
UniProt Synonym Protein Names
Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MTLRP; Ghrelin  [Similar Products]
UniProt Entry Name
GHRL_HUMAN
NCBI Summary for GHRL
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]
UniProt Comments for GHRL
ghrelin: a hormone that binds to the growth hormone secretagogue receptor type 1 (GHSR). Secreted by the stomach

Protein type: Secreted; Secreted, signal peptide; Cell cycle regulation; Apoptosis; Cell development/differentiation

Chromosomal Location of Human Ortholog: 3p26-p25

Cellular Component: axon; cytosol; endoplasmic reticulum lumen; extracellular region; extracellular space

Molecular Function: G-protein-coupled receptor binding; ghrelin receptor binding; growth hormone-releasing hormone activity; protein binding; protein tyrosine kinase activator activity

Biological Process: actin polymerization and/or depolymerization; activation of MAPK activity; adult feeding behavior; cartilage development; cortisol secretion; decidualization; dendrite development; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; gastric acid secretion; glucose metabolic process; growth hormone secretion; hormone-mediated signaling; negative regulation of angiogenesis; negative regulation of apoptosis; negative regulation of circadian sleep/wake cycle, REM sleep; negative regulation of endothelial cell proliferation; negative regulation of inflammatory response; negative regulation of insulin secretion; negative regulation of interleukin-1 beta production; negative regulation of interleukin-6 biosynthetic process; negative regulation of locomotion; negative regulation of tumor necrosis factor biosynthetic process; positive regulation of adrenocorticotropic hormone secretion; positive regulation of appetite; positive regulation of circadian sleep/wake cycle, non-REM sleep; positive regulation of cortisol secretion; positive regulation of growth hormone secretion; positive regulation of insulin secretion; positive regulation of multicellular organism growth; positive regulation of synaptogenesis; regulation of cell proliferation; regulation of excitatory postsynaptic membrane potential; regulation of response to food; response to estrogen stimulus; response to hormone stimulus

Disease: Obesity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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