• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-ENO2 antibody :: Rabbit anti-Human, rat NSE (Neuron specific enolase) Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS502094
Unit / Price
  0.1 mL  /  $315 +1 FREE 8GB USB
Testing Data
Product Name

NSE (Neuron specific enolase) (ENO2), Polyclonal Antibody

Popular Item
Also Known As

Anti-Neuron Specific Enolase (NSE)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
434
Chromosome Location
Chromosome: 12; NC_000012.12 (6914450..6923696). Location: 12p13
OMIM
131360
3D Structure
ModBase 3D Structure for P09104
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, rat
Specificity
Specific for the ~47kDa NSE protein.
Purity/Purification
Neat Serum (Unpurified neat serum.)
Form/Format
Liquid.
Antigen
Recombinant human NSE expressed in and purified from E. coli.
Immunogen Information
Recombinant human NSE expressed in and purified from E. coli
Immunogen Species
Human
Species Reactivity Note
The antibody has been directly tested for reactivity in human and rat. It is expected that it will work on other mammalian tissues.
Biological Significance
Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Other Notes
Small volumes of anti-ENO2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ENO2 antibody
Rabbit polyclonal antibody
Applications Tested/Suitable for anti-ENO2 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-ENO2 antibody
Quality Control: Western blots performed on each lot.
WB: 1:2,000
IF: 1:500

Testing Data of anti-ENO2 antibody
anti-ENO2 antibody Testing Data image
NCBI/Uniprot data below describe general gene information for ENO2. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47
NCBI Official Full Name
gamma-enolase
NCBI Official Synonym Full Names
enolase 2 (gamma, neuronal)
NCBI Official Symbol
NCBI Official Synonym Symbols
NSE; HEL-S-279
  [Similar Products]
NCBI Protein Information
gamma-enolase; neural enolase; neuron-specific enolase; neurone-specific enolase; neuron specific gamma enolase; 2-phospho-D-glycerate hydrolyase; 2-phospho-D-glycerate hydro-lyase; epididymis secretory protein Li 279
UniProt Protein Name
Gamma-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 2; Neural enolase; Neuron-specific enolase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
UniProt Entry Name
ENOG_HUMAN
NCBI Summary for ENO2
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]
UniProt Comments for ENO2
Function: Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival

By similarity.

Catalytic activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer.

Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

Subunit structure: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific.

Subcellular location: Cytoplasm

By similarity. Cell membrane

By similarity. Note: Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form

By similarity.

Tissue specificity: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

Developmental stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

Induction: Levels of ENO2 increase dramatically in cardiovascular accidents, cerebral trauma, brain tumors and Creutzfeldt-Jakob disease.

Sequence similarities: Belongs to the enolase family.

Sequence caution: The sequence AAA52388.1 differs from that shown. Reason: Erroneous initiation.
Product References and Citations for anti-ENO2 antibody
• MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). Eur J Biochem. 178(2):413-17.
• Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. J. Neurotrauma 23:1201-1210 (2006).

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000