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anti-PLK3 antibody :: Rabbit anti-Human, Rat PLK3 Polyclonal Antibody

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Catalog # MBS7045722
Unit / Price
  0.05 mL  /  $120 +1 FREE 8GB USB
  0.1 mL  /  $160 +1 FREE 8GB USB
Product Name

PLK3, Polyclonal Antibody

Popular Item
Also Known As

PLK3 Antibody

Product Synonym Names
Serine/threonine-protein kinase PLK3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Proliferation-related kinase; PLK3; CNK; FNK; PRK
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
646
OMIM
602913
3D Structure
ModBase 3D Structure for Q9H4B4
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Species
Human
Immunogen
Recombinant human Serine/threonine-protein kinase PLK3 protein (C-160AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9580 / sc-9578 / sc-25422
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PLK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PLK3 antibody
Serine/threonine-protein kinase involved in cell cycle regulation, response to stress and Golgi disassembly. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates ATF2, BCL2L1, CDC25A, CDC25C, CHEK2, HIF1A, JUN, p53/TP53, p73/TP73, PTEN, TOP2A and VRK1. Involved in cell cycle regulation: required for entry into S phase and cytokinesis. Phosphorylates BCL2L1, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Plays a key role in response to stress: rapidly activated upon stress stimulation, such as ionizing radiation, reactive oxygen species (ROS), hyperosmotic stress, UV irradiation and hypoxia. Involved in DNA damage response and G1/S transition checkpoint by phosphorylating CDC25A, p53/TP53 and p73/TP73. Phosphorylates p53/TP53 in response to reactive oxygen species (ROS), thereby promoting p53/TP53-mediated apoptosis. Phosphorylates CHEK2 in response to DNA damage, promoting the G2/M transition checkpoint. Phosphorylates the transcription factor p73/TP73 in response to DNA damage, leading to inhibit p73/TP73-mediated transcriptional activation and pro-apoptotic functions. Phosphorylates HIF1A and JUN is response to hypoxia. Phosphorylates ATF2 following hyperosmotic stress in corneal epithelium. Also involved in Golgi disassembly during the cell cycle: part of a MEK1/MAP2K1-dependent pathway that induces Golgi fragmentation during mitosis by mediating phosphorylation of VRK1. May participate in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation, via its interaction with CIB1.
Applications Tested/Suitable for anti-PLK3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Western Blot (WB) of anti-PLK3 antibody
Western blot
All lanes: PLK3 antibody at 1.2ug/ml
Lane 1: Rat heart tissue
Lane 2: Hela whole cell lysate
Lane 3: 293T whole cell lysate
Lane 4: MCF7 whole cell lysate
Lane 5: Monocytic leukemia cells in mice Macrophages
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa

anti-PLK3 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PLK3 antibody
Immunohistochemistry of paraffin-embedded human testis tissue using MBS7045722 at dilution 1:100
anti-PLK3 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-PLK3 antibody
Immunohistochemistry of paraffin-embedded human colon cancer using MBS7045722 at dilution 1:100
anti-PLK3 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PLK3. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
71,629 Da
NCBI Official Full Name
serine/threonine-protein kinase PLK3
NCBI Official Synonym Full Names
polo like kinase 3
NCBI Official Symbol
NCBI Official Synonym Symbols
CNK; FNK; PRK; PLK-3
  [Similar Products]
NCBI Protein Information
serine/threonine-protein kinase PLK3
UniProt Protein Name
Serine/threonine-protein kinase PLK3
UniProt Synonym Protein Names
Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Proliferation-related kinase
UniProt Gene Name
UniProt Synonym Gene Names
CNK; FNK; PRK; PLK-3  [Similar Products]
UniProt Entry Name
PLK3_HUMAN
NCBI Summary for PLK3
The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015]
UniProt Comments for PLK3
PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis.

Protein type: EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Nucleolus; Kinase, protein; Other group; PLK family

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: centrosome; cytoplasm; Golgi stack; nucleolus; nucleoplasm; nucleus

Molecular Function: p53 binding; protein binding; protein serine/threonine kinase activity

Biological Process: apoptosis; cytoplasmic microtubule organization and biogenesis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; endomitotic cell cycle; G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; mitotic cell cycle checkpoint; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; protein kinase B signaling cascade; regulation of cell division; regulation of cytokinesis; response to DNA damage stimulus; response to osmotic stress; response to radiation; response to reactive oxygen species
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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