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anti-PPT1 antibody :: Rabbit anti-Human, Rat PPT1 Polyclonal Antibody

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Catalog # MBS7052297
Unit / Price
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  0.05 mg  /  $160 +1 FREE 8GB USB
  0.1 mg  /  $235 +1 FREE 8GB USB
Product Name

PPT1, Polyclonal Antibody

Popular Item
Full Product Name

PPT1 Antibody

Product Synonym Names
Palmitoyl-protein thioesterase 1; PPT-1; Palmitoyl-protein hydrolase 1; PPT1; PPT
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
L42809 Genomic DNA
3D Structure
ModBase 3D Structure for P50897
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Immunogen
Recombinant Human Palmitoyl-protein thioesterase 1 protein (100-258AA)
Conjugation
Non-conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-PPT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-PPT1 antibody
Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.
Applications Tested/Suitable for anti-PPT1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-PPT1 antibody
WB: 1:500-1:5000
IHC: 1:20-1:200

Western Blot (WB) of anti-PPT1 antibody
Western Blot
Positive WB detected in: A549 whole cell lysate,HEK293 whole cell lysate,HL-60 whole cell lysate,Rat liver tissue
All lanes: PPT1 antibody at 3.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35,24 KDa
Observed band size: 35 KDa

anti-PPT1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-PPT1 antibody
Immunohistochemistry of paraffin-embedded human ovarian cancer using MBS7052297 at dilution of 1:100
anti-PPT1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for PPT1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
23,094 Da
NCBI Official Full Name
palmitoyl-protein thioesterase 1 isoform 1
NCBI Official Synonym Full Names
palmitoyl-protein thioesterase 1
NCBI Official Symbol
NCBI Official Synonym Symbols
PPT; CLN1; INCL
  [Similar Products]
NCBI Protein Information
palmitoyl-protein thioesterase 1
UniProt Protein Name
Palmitoyl-protein thioesterase 1
UniProt Synonym Protein Names
Palmitoyl-protein hydrolase 1
UniProt Gene Name
UniProt Synonym Gene Names
; PPT-1  [Similar Products]
NCBI Summary for PPT1
The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008]
UniProt Comments for PPT1
PPT1: Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons. Defects in PPT1 are the cause of neuronal ceroid lipofuscinosis type 1 (CLN1). A form of neuronal ceroid lipofuscinosis with variable age at onset. Infantile, late- infantile, juvenile, and adult onset have been reported. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. The lipopigment pattern seen most often in CLN1 is referred to as granular osmiophilic deposits (GROD). Belongs to the palmitoyl-protein thioesterase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.2.22; Hydrolase; Lipid Metabolism - fatty acid elongation in mitochondria

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: axon; cytosol; extracellular region; Golgi apparatus; lipid raft; lysosomal lumen; lysosome; membrane; nucleus; synaptic vesicle

Molecular Function: palmitoyl-(protein) hydrolase activity; palmitoyl-CoA hydrolase activity; protein binding

Biological Process: brain development; cofactor metabolic process; cofactor transport; lipid catabolic process; lipid raft organization and biogenesis; lysosomal lumen acidification; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of neuron apoptosis; nervous system development; neuron development; pinocytosis; positive regulation of pinocytosis; positive regulation of receptor-mediated endocytosis; protein depalmitoylation; protein transport; receptor-mediated endocytosis; sphingolipid catabolic process; synaptic transmission

Disease: Ceroid Lipofuscinosis, Neuronal, 1
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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