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anti-M6PRBP1 antibody :: Rabbit anti-Human, Rat TIP47 Polyclonal Antibody

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Catalog # MBS151454
Unit / Price
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  0.1 mg  /  $375 +1 FREE 8GB USB
Product Name

TIP47 (M6PRBP1), Polyclonal Antibody

Full Product Name

TIP47 Antibody

Product Synonym Names
TIP47; PP17; TIP47; M6PRBP1; Perilipin-3; 47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; mannose-6-phosphate receptor binding protein 1
Antibody/Peptide Pairs
TIP47 peptide (MBS152707) is used for blocking the activity of TIP47 antibody (MBS151454)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602702
3D Structure
ModBase 3D Structure for O60664
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat
Purity/Purification
TIP47 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (lot specific)
Conjugate
Unconjugated
Immunogen
TIP47 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human TIP47.
Buffer
TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TIP47 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Other Notes
Small volumes of anti-M6PRBP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-M6PRBP1 antibody
TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
Applications Tested/Suitable for anti-M6PRBP1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes for anti-M6PRBP1 antibody
TIP47 antibody can be used for the detection of TIP47 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.

Western Blot (WB) of anti-M6PRBP1 antibody
Western blot analysis of TIP47 in Daudi cell lysate with TIP47 antibody at (A) 0.5 and (B) 1 μg/mL.
anti-M6PRBP1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-M6PRBP1 antibody
Immunohistochemistry of TIP47 in rat liver tissue with TIP47 antibody at 10 μg/mL.
anti-M6PRBP1 antibody Immunohistochemistry (IHC) (IHC) image
Immunofluorescence (IF) of anti-M6PRBP1 antibody
Immunofluorescence of TIP47 in Rat Liver cells with TIP47 antibody at 20 μg/mL.
anti-M6PRBP1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for M6PRBP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
45,803 Da
NCBI Official Full Name
Perilipin-3
NCBI Official Synonym Full Names
perilipin 3
NCBI Official Symbol
PLIN3  [Similar Products]
NCBI Official Synonym Symbols
PP17; TIP47; M6PRBP1
  [Similar Products]
NCBI Protein Information
perilipin-3; placental protein 17; 47 kDa MPR-binding protein; cargo selection protein TIP47; tail-interacting protein, 47 kD; mannose-6-phosphate receptor-binding protein 1
UniProt Protein Name
Perilipin-3
UniProt Synonym Protein Names
47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; Mannose-6-phosphate receptor-binding protein 1; Placental protein 17; PP17
UniProt Gene Name
PLIN3  [Similar Products]
UniProt Synonym Gene Names
M6PRBP1; TIP47; 47 kDa MPR-binding protein; PP17  [Similar Products]
UniProt Entry Name
PLIN3_HUMAN
NCBI Summary for M6PRBP1
Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]
UniProt Comments for M6PRBP1
perilipin 3: a membrane-associated protein and a coat protein for lipid droplets (LDs). It has been described as a mannose-6-phosphate receptor (MPR) binding protein required for endosome-to-Golgi transport of MPRs, but this function has been disputed. Interacts with M6PR and IGF2R via their cytoplasmic domains. Affects hepatic lipid and glucose metabolism. Belongs to the perilipin family. 3 isoforms of the human protein are produced by alternative splicing. Isoform 2 may exist as a homodimer known as PP17C.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; cytoplasm; endosome membrane; lipid particle; endosome

Molecular Function: protein binding

Biological Process: vesicle-mediated transport
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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