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Rabbit anti-Human, Rat WASP, N-, non-phosphorylated Polyclonal Antibody

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Catalog # MBS616068
Unit / Price
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  0.1 mL  /  $600 +1 FREE 8GB USB
anti-WASP, N-, non-phosphorylated antibody
Product Name

WASP, N-, non-phosphorylated, Polyclonal Antibody

Full Product Name

WASP, N-, non-phosphorylated (Ser-484/Ser-485) (WAS, THC, IMD2, WASP, Eczema-thrombocytopenia)

Product Synonym Names
Anti -WASP, N-, non-phosphorylated (Ser-484/Ser-485) (WAS, THC, IMD2, WASP, Eczema-thrombocytopenia)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Rat
Specificity
Cross-absorbed to phospho-N-WASP (Ser-384/Ser-385) then affinity purified using nonphospho-N-WASP (Ser-384/Ser-385) peptide. The antibody detects a 65 kDa protein on SDS-PAGE immunoblots of rat brain and SKN-SH cell lysates that are treated with alkaline phosphatase. Only low levels of unphosphorylated WASP or N-WASP are detected in untreated rat brain, Jurkat, and A431 cells.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Immunogen
Nonphospho-N-WASP (Ser-384/Ser-385) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around serine 384 and 385 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 383 and 384.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-WASP, N-, non-phosphorylated antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-WASP, N-, non-phosphorylated antibody
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3, which can nucleate actin polymerization at sites that lead to branched actin structures. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63kD protein in hematopoietic cells, while N-WASP is observed as a 65kD in many tissues, especially brain.

Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. These clinical findings as well as the structural properties of WASP (including a cdc42 binding site, SH3 domain binding region and regions for actin cytoskeletal localization) suggest a pivotal role for WASP in regulating the structure and function of platelets and T-lymphocytes. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.
Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 383 and 384 by casein kinases increase the affinity for the Arp2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types.
Applications Tested/Suitable for anti-WASP, N-, non-phosphorylated antibody
ELISA (EL/EIA), Western Blot (WB)
Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols.
NCBI/Uniprot data below describe general gene information for WASP, N-, non-phosphorylated. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Official Full Name
Wiskott-Aldrich syndrome protein
NCBI Summary for WASP, N-, non-phosphorylated
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]
UniProt Comments for WASP, N-, non-phosphorylated
WASP: a member of the Wiskott-Aldrich syndrome (WAS) family of proteins. A cytoplasmic protein expressed exclusively in hematopoietic cells. Transduces signals from surface receptors to the actin cytoskeleton. Associates with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Mutated in Wiskott-Aldrich syndrome, a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.4-p11.21

Cellular Component: intercellular junction; vesicle membrane; cytosol; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; phospholipase binding; actin binding; protein kinase binding; SH3 domain binding

Biological Process: epidermis development; T cell activation; actin filament-based movement; defense response; endosome transport; T cell receptor signaling pathway; actin filament polymerization; regulation of catalytic activity; actin polymerization and/or depolymerization; innate immune response; immune response; protein complex assembly; blood coagulation

Disease: Thrombocytopenia 1; Neutropenia, Severe Congenital, X-linked
Precautions
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Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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