View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Add To Compare List Rabbit anti-Mammals Phospho Prion Protein Polyclonal Antibody Catalog # MBS588229 Unit / Price DISCONTINUEDClick here to view our similar product(s) OR click here to contact our technical support team for suitable replacement. Previous Next Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name Phospho Prion Protein, Polyclonal Antibody Full Product Name Phospho Prion Protein (pPrP Ser43) Rabbit Polyclonal Antibody Product Synonym Names anti-Phospho Prion Protein; anti-Prion Protein; PrP; ASCR; PrP27-30; PrP33-35C Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Rabbit Species Reactivity Mammals Specificity A highly specific and sensitive antibody against the phosphorylation of the Ser43 of the Prion Protein. The antibody reacts with the Ser43 (G[pSer]PGGNRYPC) of the Phosphorylated Prion Protein. Form/Format Lyophilized serum Immunogen Phospho-peptide Preservative None Preparation and Storage Lyophilized antibody can be kept at 4 degree C for up to 3 months and should be kept at-20 degree C for long-term storage.To avoid freeze-thaw cycles, reconstituted antibody should be aliquoted before freezing for short-term storage (-20 degree C) or for long-term storage (-80 degree C).For maximum recovery of product centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Other Notes Small volumes of anti-Phospho Prion Protein antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-Phospho Prion Protein antibody Prion related neurodegenerative diseases, called transmissible spongiform encephalopathies, are observed in many animal species. These diseases involve conversion of normal cellular prion protein (PrPc) into a form that is insoluble and resistant to proteases (PrPSc). The protease resistant form can polymerize into fibrils which accumulate in infected tissues and cause cell death and tissue damage. PrPs have an N-terminal signal sequence and a C-terminal linkage to glycosylphosphatidylinositol anchor. The mature protein is a glycosylated protein that associates with cell membranes. Phosphorylation of PrPC at Ser-43 by Cdk5 promotes proteinase K resistance, prion aggregation, and fibril formation in vitro. In addition, Ser-43 phosphorylation is upregulated in scrapie-infected mouse brain relative to controls. Thus, phosphorylation of Ser-43 may be an important mechanism leading conversion of PrPc to PrPSc and the onset of disease. The antibody was raised against Phospho Prion Protein (pPrP Ser43). Applications Tested/Suitable for anti-Phospho Prion Protein antibody ELISA (EIA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB) Application Notes for anti-Phospho Prion Protein antibody If reconstituted in 50 muL: ELISA (phospho peptide) 1:500,000; ELISA (non-phospho peptide) 1:25,000 WB: 1:100; IP 1:100; IHC: 1:200-1:1000. Western Blot (WB) of anti-Phospho Prion Protein antibody Antibody specificity was tested by Western blot analysis in non-phosphorylated PrP and Cdk5-phosphorylated Prp; using a monoclonal antibody against the Prion Protein (3F4) and the anti-pPrPS43 Western Blot (WB) of anti-Phospho Prion Protein antibody Antibody specificity was tested by Western blot analysis using an antibody against the Prion Protein (3F4) of immunoprecipitated PrP and pPrP immunoprecipitated with anti-pPrPS43 (MM-0112) or anti-PrP36-56 R155 polyclonal antibody (which was generated against residues 36-56 of full length WT SHaPrP TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP