• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-S17aH antibody :: Rabbit anti-Mouse 17-Alpha-Hydroxylase Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS2033993 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $115 +1 FREE 8GB USB
  0.02 mg  /  $135 +1 FREE 8GB USB
  0.05 mg  /  $195 +1 FREE 8GB USB
  0.1 mg  /  $250 +1 FREE 8GB USB
  0.2 mg  /  $380 +1 FREE 8GB USB
  1 mg  /  $905 +1 FREE 8GB USB
Product Name

17-Alpha-Hydroxylase (S17aH), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to 17-Alpha-Hydroxylase (S17aH)

Product Synonym Names
CYP17A1; CPT7; P450C17; 17-alpha-hydroxyprogesterone aldolase/17,20-Lyase; Steroid 17-alpha-monooxygenase; Cytochrome P450,Family 17,Subfamily A,Polypeptide 1
Matching Pairs
Matching Pairs
Unconjugated Antibody: 17-Alpha-Hydroxylase (MBS2033993)
APC-CY7 Conjugated Antibody: 17-Alpha-Hydroxylase (S17aH) (MBS2062101)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P27786
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against S17aH. It has been selected for its ability to recognize S17aH in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Fragment
S17aH (Thr201~Thr507)
Conjugate
No Conjugate
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant S17aH (Thr201~Thr507) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2062103
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-S17aH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-S17aH antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-S17aH antibody
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot (WB) of anti-S17aH antibody
Western Blot: Sample: Rat Testis lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse S17aH Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-S17aH antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-S17aH antibody
Western Blot: Sample: Recombinant protein.
anti-S17aH antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-S17aH antibody
DAB staining on fromalin fixed paraffin- embedded kidney tissue)
anti-S17aH antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-S17aH antibody
DAB staining on IHC-P; Samples: Mouse Testis Tissue)
anti-S17aH antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for S17aH. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
57,638 Da
NCBI Official Full Name
steroid 17-alpha-hydroxylase/17,20 lyase
NCBI Official Synonym Full Names
cytochrome P450, family 17, subfamily a, polypeptide 1
NCBI Official Symbol
Cyp17a1  [Similar Products]
NCBI Official Synonym Symbols
Cyp17; p450c17
  [Similar Products]
NCBI Protein Information
steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Protein Name
Steroid 17-alpha-hydroxylase/17,20 lyase
UniProt Synonym Protein Names
17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17
UniProt Gene Name
Cyp17a1  [Similar Products]
UniProt Synonym Gene Names
Cyp17; Cytochrome P450c17  [Similar Products]
UniProt Comments for S17aH
CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: EC 1.14.99.9; EC 4.1.2.30; Lipid Metabolism - C21-steroid hormone; Oxidoreductase

Chromosomal Location of Human Ortholog: 19 C3|19 38.97 cM

Cellular Component: axon; cell projection; cell soma; endoplasmic reticulum; intracellular membrane-bound organelle; membrane; mitochondrion

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; heme binding; iron ion binding; lyase activity; metal ion binding; monooxygenase activity; oxidoreductase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; steroid 17-alpha-monooxygenase activity

Biological Process: glucocorticoid biosynthetic process; hormone biosynthetic process; progesterone metabolic process; steroid biosynthetic process; steroid metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000