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anti-HSD17b12 antibody :: Rabbit anti-Mouse 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12) Polyclonal Antibody

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Catalog # MBS2005227 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $185 +1 FREE 8GB USB
  0.1 mg  /  $230 +1 FREE 8GB USB
  0.2 mg  /  $345 +1 FREE 8GB USB
  1 mg  /  $825 +1 FREE 8GB USB
Product Name

17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to 17-Beta-Hydroxysteroid Dehydrogenase Type 12 (HSD17b12)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -MECAPPAAG F LYWVGASTIA YLALRASYSL FRAFQVWCVG NEALVGPRLG EWAVVTGGTD GIGKAYAEEL AKRGMKIVLI SRSQDKLNQV SNNIKEKFNV ETRTIAVDFS LDDIYDKIKT GLSGLEIGVL VNNVGMSYEY PEYFLEIPDL DNTIKKLINI NVLSVCKVTR LVLPGMVERS KGVILNISSA SGMLPVPLLT IYSATKAFVD FFSQCLHEEY KSKGIFVQSV MPYLVATKLA KIQKPTLDKP SAETFVKSAI KTVGLQTRTT GYVIHSLMGS INSIMPRWMY FKIIMGFSKS LRNRYLKKRK KN
3D Structure
ModBase 3D Structure for O70503
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against HSD17b12. It has been selected for its ability to recognize HSD17b12 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant HSD17b12 (Met1~Asn312) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2076053
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-HSD17b12 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HSD17b12 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-HSD17b12 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-HSD17b12 antibody
Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HSD17b12 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-HSD17b12 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-HSD17b12 antibody
Western Blot: Sample: Recombinant protein.
anti-HSD17b12 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-HSD17b12 antibody
DAB staining on fromalin fixed paraffin- embedded kidney tissue)
anti-HSD17b12 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-HSD17b12 antibody
DAB staining on IHC-P; Samples: Mouse Lymph node Tissue)
anti-HSD17b12 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for HSD17b12. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
20,127 Da
NCBI Official Full Name
very-long-chain 3-oxoacyl-CoA reductase
NCBI Official Synonym Full Names
hydroxysteroid (17-beta) dehydrogenase 12
NCBI Official Symbol
Hsd17b12  [Similar Products]
NCBI Official Synonym Symbols
Kik1; KIK-I; AI172963; 2610510O05Rik
  [Similar Products]
NCBI Protein Information
very-long-chain 3-oxoacyl-CoA reductase
UniProt Protein Name
Very-long-chain 3-oxoacyl-CoA reductase
UniProt Synonym Protein Names
17-beta-hydroxysteroid dehydrogenase 12
UniProt Gene Name
Hsd17b12  [Similar Products]
UniProt Synonym Gene Names
UniProt Comments for HSD17b12
HSD17B12: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Protein type: EC 1.1.1.62; Lipid Metabolism - androgen and estrogen; Lipid Metabolism - unsaturated fatty acid biosynthesis; Membrane protein, integral; Membrane protein, multi-pass; Oxidoreductase

Chromosomal Location of Human Ortholog: 2|2 E1

Cellular Component: endoplasmic reticulum; integral component of membrane; membrane; proteinaceous extracellular matrix

Molecular Function: collagen binding; estradiol 17-beta-dehydrogenase activity; fibronectin binding; heparin binding; long-chain-3-hydroxyacyl-CoA dehydrogenase activity; oxidoreductase activity

Biological Process: extracellular matrix organization; lipid metabolic process; steroid biosynthetic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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