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anti-ACAA1 antibody :: Rabbit anti-Mouse Acetyl Coenzyme A Acyltransferase 1 Polyclonal Antibody

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Catalog # MBS2033848 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $185 +1 FREE 8GB USB
  0.1 mg  /  $230 +1 FREE 8GB USB
  0.2 mg  /  $345 +1 FREE 8GB USB
  1 mg  /  $825 +1 FREE 8GB USB
Product Name

Acetyl Coenzyme A Acyltransferase 1 (ACAA1), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to Acetyl Coenzyme A Acyltransferase 1 (ACAA1)

Product Synonym Names
ACAA; PTHIO; Peroxisomal 3-Oxoacyl-Coenzyme A Thiolase; Acetyl-CoA acyltransferase; Beta-ketothiolase
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q921H8
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against ACAA1. It has been selected for its ability to recognize ACAA1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Fragment
ACAA1 (Gly182~Asn424)
Conjugate
No Conjugate
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant ACAA1 (Gly182~Asn424) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2072387
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-ACAA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ACAA1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-ACAA1 antibody
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot (WB) of anti-ACAA1 antibody
Western Blot: Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse ACAA1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-ACAA1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-ACAA1 antibody
Western Blot: Sample: Recombinant protein.
anti-ACAA1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ACAA1 antibody
DAB staining on fromalin fixed paraffin- embedded liver tissue)
anti-ACAA1 antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-ACAA1 antibody
DAB staining on IHC-P; Samples: Mouse Liver Tissue)
anti-ACAA1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for ACAA1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
43,953 Da
NCBI Official Full Name
3-ketoacyl-CoA thiolase A, peroxisomal isoform 1
NCBI Official Synonym Full Names
acetyl-Coenzyme A acyltransferase 1A
NCBI Official Symbol
Acaa1a  [Similar Products]
NCBI Official Synonym Symbols
PTL; Acaa; Acaa1; D9Ertd25e
  [Similar Products]
NCBI Protein Information
3-ketoacyl-CoA thiolase A, peroxisomal
UniProt Protein Name
3-ketoacyl-CoA thiolase A, peroxisomal
UniProt Synonym Protein Names
Acetyl-CoA acyltransferase A; Beta-ketothiolase A; Peroxisomal 3-oxoacyl-CoA thiolase A
UniProt Gene Name
Acaa1a  [Similar Products]
UniProt Synonym Gene Names
Acaa1  [Similar Products]
UniProt Comments for ACAA1
ACAA1: an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Protein type: Acetyltransferase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 2.3.1.16; Lipid Metabolism - fatty acid; Lipid Metabolism - unsaturated fatty acid biosynthesis

Chromosomal Location of Human Ortholog: 9 F3|9 71.33 cM

Cellular Component: intracellular membrane-bound organelle; membrane; mitochondrion; peroxisome

Molecular Function: acetate CoA-transferase activity; acetyl-CoA C-acetyltransferase activity; acetyl-CoA C-acyltransferase activity; catalytic activity; palmitoyl-CoA oxidase activity; transferase activity; transferase activity, transferring acyl groups; transferase activity, transferring groups other than amino-acyl groups

Biological Process: bile acid metabolic process; fatty acid beta-oxidation; fatty acid metabolic process; lipid metabolic process; metabolic process; very long-chain fatty acid metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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