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anti-AST antibody :: Rabbit anti-Mouse Aspartate Aminotransferase (AST) Polyclonal Antibody

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Catalog # MBS2005679 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
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  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $330 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
Product Name

Aspartate Aminotransferase (AST), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to Aspartate Aminotransferase (AST)

Product Gene Name
Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG S-MAPPSVFA QV PQAPPVLVFK LTADFRDDPD PRKVNLGVGA YRTDESQPWV LPVVRKVEQK IANDNSLNHE YLPILGLAEF RSCASRLVLG DNSLAIRENR VGGVQSLGGT GALRIGADFL GRWYNGTDNK NTPIYVSSPT WENHNAVFSA AGFKDIRPYC YWDAEKRGLD LQGFLNDLEN APEFSIFVLH ACAHNPTGTD PTPEQWKQIA AVMQRRFLFP FFDSAYQGFA SGDLEKDAWA IRYFVSEGFE LFCAQSFSKN FGLYNERVGN LTVVGKESDS VLRVLSQMEK IVRITWSNPP AQGARIVAAT LSDPELFKEW KGNVKTMADR ILTMRSELRA RLEALKTPGT WSHITEQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINM CGLTTKNLDY VATSIHEAVT KIQ
3D Structure
ModBase 3D Structure for P05201
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against AST. It has been selected for its ability to recognize AST in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant AST (Met1~Gln413) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2052593
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-AST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-AST antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-AST antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-AST antibody
Western Blot: Sample: Recombinant protein.
anti-AST antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-AST antibody
DABstainingonIHC-P.Samples:MouseTissue)
anti-AST antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for AST. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
46,248 Da
NCBI Official Full Name
aspartate aminotransferase, cytoplasmic
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 1, soluble
NCBI Official Symbol
NCBI Official Synonym Symbols
cCAT; Got-1; cAspAT; AI789014
  [Similar Products]
NCBI Protein Information
aspartate aminotransferase, cytoplasmic
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
cAspAT; cCAT  [Similar Products]
UniProt Comments for AST
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase

Chromosomal Location of Human Ortholog: 19 C3|19 36.67 cM

Cellular Component: axon terminus; cytoplasm; cytosol; extracellular exosome; lysosome; nucleoplasm; nucleus

Molecular Function: carboxylic acid binding; catalytic activity; L-aspartate:2-oxoglutarate aminotransferase activity; L-cysteine:2-oxoglutarate aminotransferase activity; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding; transaminase activity; transferase activity

Biological Process: amino acid biosynthetic process; amino acid metabolic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; biosynthetic process; dicarboxylic acid metabolic process; fatty acid homeostasis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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