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anti-ASXL1 antibody :: Rabbit anti-Mouse ASXL1 Polyclonal Antibody

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Catalog # MBS9211148
Unit / Price
  0.08 mL  /  $150 +1 FREE 8GB USB
  0.4 mL  /  $340 +1 FREE 8GB USB
Product Name

ASXL1, Polyclonal Antibody

Also Known As

ASXL1 Antibody (Center)

Product Synonym Names
Putative Polycomb group protein ASXL1; Additional sex combs-like protein 1; ASXL1; KIAA0978
Antibody/Peptide Pairs
ASXL1 peptide (MBS9218168) is used for blocking the activity of ASXL1 antibody (MBS9211148)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
521-549
Immunogen Sequence Length
479
OMIM
605039
3D Structure
ModBase 3D Structure for Q8IXJ9
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Mouse
Specificity
This ASXL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-549 amino acids from the Central region of human ASXL1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.43 (lot specific)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-ASXL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ASXL1 antibody
This gene is similar to the Drosophila additional sex
combs gene, which encodes a chromatin-binding protein required for
normal determination of segment identity in the developing embryo.
The protein is a member of the Polycomb group of proteins, which
are necessary for the maintenance of stable repression of homeotic
and other loci. The protein is thought to disrupt chromatin in
localized areas, enhancing transcription of certain genes while
repressing the transcription of other genes. The protein encoded by
this gene functions as a ligand-dependent co-activator for retinoic
acid receptor in cooperation with nuclear receptor coactivator 1.
Mutations in this gene are associated with myelodysplastic
syndromes and chronic myelomonocytic leukemia. Alternative splicing
results in multiple transcript variants.
Product Categories/Family for anti-ASXL1 antibody
Applications Tested/Suitable for anti-ASXL1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-ASXL1 antibody
WB~~1:1000

Western Blot (WB) of anti-ASXL1 antibody
ASXL1 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ASXL1 antibody detected the ASXL1 protein (arrow).
anti-ASXL1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-ASXL1 antibody
ASXL1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ASXL1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-ASXL1 antibody Immunohistochemistry (IHC) (IHC) image
Flow Cytometry (FC/FACS) of anti-ASXL1 antibody
ASXL1 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
anti-ASXL1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
Immunofluorescence (IF) of anti-ASXL1 antibody
Confocal immunofluorescent analysis of ASXL1 Antibody (Center) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
anti-ASXL1 antibody Immunofluorescence (IF) image
NCBI/Uniprot data below describe general gene information for ASXL1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
165448
NCBI Official Full Name
putative Polycomb group protein ASXL1 isoform 1
NCBI Official Synonym Full Names
additional sex combs like transcriptional regulator 1
NCBI Official Symbol
ASXL1  [Similar Products]
NCBI Official Synonym Symbols
MDS; BOPS
  [Similar Products]
NCBI Protein Information
putative Polycomb group protein ASXL1
UniProt Protein Name
Putative Polycomb group protein ASXL1
UniProt Synonym Protein Names
Additional sex combs-like protein 1
UniProt Gene Name
ASXL1  [Similar Products]
UniProt Synonym Gene Names
KIAA0978  [Similar Products]
UniProt Entry Name
ASXL1_HUMAN
NCBI Summary for ASXL1
This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
UniProt Comments for ASXL1
ASXL1: Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG). Acts as coactivator of RARA and RXRA through association with NCOA1. Acts as corepressor through recruitment of KDM1A and CBX5 to target genes in a cell-type specific manner; the function seems to involve differential recruitment of methylated histone H3 to respective promoters. Acts as corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity. Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Defects in ASXL1 are the cause of Bohring-Opitz syndrome (BOPS). A syndrome characterized by severe intrauterine growth retardation, poor feeding, profound mental retardation, trigonocephaly, prominent metopic suture, exophthalmos, nevus flammeus of the face, upslanting palpebral fissures, hirsutism, and flexion of the elbows and wrists with deviation of the wrists and metacarpophalangeal joints. Defects in ASXL1 are a cause of myelodysplastic syndrome (MDS). A heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS); chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. MDS is considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Belongs to the Asx family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 20q11

Cellular Component: nuclear chromatin

Molecular Function: peroxisome proliferator activated receptor binding; protein binding; DNA binding; retinoic acid receptor binding; metal ion binding; transcription coactivator activity; transcription corepressor activity

Biological Process: negative regulation of retinoic acid receptor signaling pathway; response to retinoic acid; transcription, DNA-dependent; positive regulation of retinoic acid receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation

Disease: Bohring-opitz Syndrome; Myelodysplastic Syndrome
Product References and Citations for anti-ASXL1 antibody
Abdel-Wahab, O., et al. Leukemia 24(9):1656-1657(2010)
Szpurka, H., et al. Leuk. Res. 34(8):969-973(2010)
Sugimoto, Y., et al. Br. J. Haematol. 150(1):83-87(2010)
Boultwood, J., et al. Leukemia 24(6):1139-1145(2010)
Rocquain, J., et al. BMC Cancer 10, 401 (2010) :

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Disclaimer
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