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anti-MET antibody :: Rabbit anti-Mouse. c-Met Polyclonal Antibody

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Catalog # MBS629762
Unit / Price
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  0.05 mg  /  $670 +1 FREE 8GB USB
Product Name

c-Met, Polyclonal Antibody

Popular Item
Full Product Name

c-Met, phosphorylated (Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)

Product Synonym Names
Anti -c-Met, phosphorylated (Tyr1234, Tyr1235) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 14; NC_006596.2 (58591076..58674517)
3D Structure
ModBase 3D Structure for Q75ZY9
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse.
Specificity
Detects human c-MET phosphorylated at Y1234/Y1235.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution containing PBS, pH 7.4, 5% trehalose. Reconstitute with 250 ul sterile PBS.
Concentration
~0.2 mg/mL(after reconstitution) (lot specific)
Immunogen
Phosphopeptide containing human HGF R/c-MET Y1234/1235 sites
Preparation and Storage
Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-MET antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-MET antibody
Hepatocyte growth factor receptor (HGF R), also known as c-MET, is a receptor tyrosine kinase that consists of an extracellular chain and a longer chain. The chain contains the transmembrane helix and the cytoplasmic portion. Ligand-stimulated phosphorylation of tyrosine 1234 and tyrosine 1235 in HGF R's chain results in stimulation of intrinsic kinase activity.
Applications Tested/Suitable for anti-MET antibody
Flow Cytometry, Immunohistochemistry,Western Blot.

Other applications not tested.
Application Notes for anti-MET antibody
Flow Cytometry: 2.5ug/1x10e6 cells using MCF-7 human breast cancer cell line treated with pervanadate, fixed with paraformaldehyde and permealized with methanol.

Immunohistochemistry: 5-15ug/ml using immersion fixed, frozen section of mouse embryo.

Western Blot: 0.5ug/ml using pervanadate-treated MDA-MB-468 human breast cancer cell line .

Optimal dilutions should be determined by each laboratory for each application.

Flow Cytometry FC of anti-MET antibody
Flow Cytometry: MCF-7 human breast cancer cell line was unstimulated (light orange open histogram) or treated with 100uM pervanadate for 10 mins.(dark orange filled histogram), then stained with MBS629762 or control antibody (blue open histogram), followed by PE-conjugated anti-rabbit IgG secondary antibody. To facilitate intracellular staining , cells with fixed with paraformaldehyde and permeabilized with methanol.
anti-MET antibody Flow Cytometry FC (FC/FACS) image
Immunohistochemical IHC of anti-MET antibody
Immunohistochemical staining of immersion fixed frozen sections of mouse embryo(13 d.p.c) using MBS629762 at 15 ug/mL(overnight incubation at 4°C . Tissue was stained using an anti-rabbit HRP-DAB staining kit and counterstained with hematoxylin (blue)
anti-MET antibody Immunohistochemical IHC image
Western Blot WB of anti-MET antibody
Western blot shows immunoprecipitate of MDA-MB-468 human breast cancer line untreated (-) or treated (+) with 100uM pervanadate for 10 mins.PVDF membrene was probed with 0.5 ug/mL MBS629762,followed by HRP-conjugated anti-rabbit IgG secondary antibody. A specific band was detected for phospho HGF R/c Met(Y1234/Y1235) at~145kD. Experiment was conducted under reducing conditions.
anti-MET antibody Western Blot WB (WB) image
NCBI/Uniprot data below describe general gene information for MET. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
154,637 Da[Similar Products]
NCBI Official Full Name
c-Met
NCBI Official Symbol
NCBI Official Synonym Symbols
c-Met
  [Similar Products]
NCBI Protein Information
hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
UniProt Protein Name
Hepatocyte growth factor receptor
UniProt Synonym Protein Names
HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met
Protein Family
UniProt Gene Name
UniProt Entry Name
MET_CANFA
UniProt Comments for MET
Function: Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Heterodimer formed of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1 and GRB2. Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1357, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10

Subcellular location: Membrane; Single-pass type I membrane protein

Domain: The kinase domain is involved in SPSB1 binding

Post-translational modification: Dephosphorylated by PTPRJ at Tyr-1350 and Tyr-1366

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family.Contains 3 IPT/TIG domains.Contains 1 protein kinase domain.Contains 1 Sema domain.
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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