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anti-CD163 antibody :: Rabbit anti-Mouse Cluster Of Differentiation (CD163) Polyclonal Antibody

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Catalog # MBS2002979 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $330 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
Product Name

Cluster Of Differentiation (CD163), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Cluster Of Differentiation (CD163)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-PVQ T TSSTIPKESE VPCIASGQLR LVGGGGRCAG RVEVYHEGSW GTVCDDNWDM TDANVVCKQL DCGVAINATG SAYFGEGAGA IWLDEVICTG KESHIWQCHS HGWGRHNCRH KEDAGVICSE FMSLRLTNEA HKENCTGRLE VFYNGTWGSI GSSNMSPTTV GVVCRQLGCA DNGTVKPIPS DKTPSRPMWV DRVQCPKGVD TLWQCPSSPW KQRQASPSSQ ESWIICDNKI RLQEGHTDCS GRVEIWHKGS WGTVCDDSWD LNDAKVVCKQ LG
3D Structure
ModBase 3D Structure for Q2VLH6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against CD163. It has been selected for its ability to recognize CD163 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant CD163 (Pro697~Gly972) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli.
Accession No.
RPB726Mu01
Buffer
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Quality Control
Content: The quality control contains recombinant C0163 (Pro697-Gly972) disposed in loading buffer.
Usage: 10ul per well when 3,3'-Oiaminobenzidine(OAB) as the substrate.
5ul per well when used in enhanced chemilumescent (ECl).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pHB.B), 2% SOS, 200mM NaCI, 50% glycerol, BPB 0.01 %, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2058317
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-CD163 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-CD163 antibody
WB, ICC, IHC-P, IHC-F, ELISA
Application Notes for anti-CD163 antibody
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-CD163 antibody
Western Blot: Sample: Recombinant protein.
anti-CD163 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-CD163 antibody
DABstainingonIHC-P.Samples:MouseTissue)
anti-CD163 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for CD163. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
125,292 Da
NCBI Official Full Name
scavenger receptor cysteine-rich type 1 protein M130 isoform 2
NCBI Official Synonym Full Names
CD163 antigen
NCBI Official Symbol
Cd163  [Similar Products]
NCBI Official Synonym Symbols
CD163v2; CD163v3
  [Similar Products]
NCBI Protein Information
scavenger receptor cysteine-rich type 1 protein M130
UniProt Protein Name
Scavenger receptor cysteine-rich type 1 protein M130
UniProt Synonym Protein Names
CD_antigen: CD163
UniProt Gene Name
Cd163  [Similar Products]
UniProt Synonym Gene Names
M130; sCD163  [Similar Products]
UniProt Comments for CD163
CD163: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6|6 F2

Cellular Component: external side of plasma membrane; extracellular region; integral component of membrane; membrane; plasma membrane

Molecular Function: scavenger receptor activity

Biological Process: acute-phase response; inflammatory response
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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