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anti-NNE antibody :: Rabbit anti-Mouse Enolase, Non Neuronal (NNE) Polyclonal Antibody

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Catalog # MBS2004811 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $175 +1 FREE 8GB USB
  0.1 mg  /  $225 +1 FREE 8GB USB
  0.2 mg  /  $330 +1 FREE 8GB USB
  1 mg  /  $785 +1 FREE 8GB USB
Product Name

Enolase, Non Neuronal (NNE), Polyclonal Antibody

Popular Item
Also Known As

Polyclonal Antibody to Enolase, Non Neuronal (NNE)

Product Gene Name
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Matching Pairs
Unconjugated Antibody: Enolase, Non Neuronal (NNE) (MBS2004811)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
Matching Pairs
Unconjugated Antibody: Enolase, Non Neuronal (NNE) (MBS2004811)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG SEFELRRQAC -MSILRIHAR E IFDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DNDKTRFMGK GVSQAVEHIN KTIAPALVSK KVNVVEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN PEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEALELLKTA IAKAGYTDQV VIGMDVAASE FYRSGKYDLD FKSPDDPSRY ITPDQLADLY KSFVQNYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AASEKSCNCL LLKVNQIGSV TESLQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQI LRIEEELGSK AKFAGRSFRN PLAK
3D Structure
ModBase 3D Structure for P17182
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against NNE. It has been selected for its ability to recognize NNE in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant NNE (Met1~Lys434) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2055320
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Other Notes
Small volumes of anti-NNE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-NNE antibody
Applications Tested/Suitable for anti-NNE antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-NNE antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) Knockout Verification of anti-NNE antibody
Knockout Verification: Lane 1: Wild-type MCF7 cell lysate
Lane 2: NNE knockout MCF7 cell lysate
Predicted MW: 36,47kDa ;Observed MW: 50kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse NNE Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-NNE antibody Western Blot (WB) Knockout Verification (WB) image
Western Blot (WB) of anti-NNE antibody
Western Blot; Sample: Mouse Placenta lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse NNE Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-NNE antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-NNE antibody
Western Blot; Sample: Recombinant protein.
anti-NNE antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-NNE antibody
DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-NNE antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-NNE antibody
DAB staining on IHC-P; Samples: Mouse Liver Tissue)
anti-NNE antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for NNE. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
Molecular Weight
47,141 Da
NCBI Official Full Name
enolase 1B, retrotransposed
NCBI Official Synonym Full Names
enolase 1B, retrotransposed
NCBI Official Symbol
Eno1b  [Similar Products]
NCBI Official Synonym Symbols
Eno1; Gm5506; EG433182
  [Similar Products]
NCBI Protein Information
enolase 1B, retrotransposed
UniProt Protein Name
Alpha-enolase
UniProt Synonym Protein Names
2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE
UniProt Gene Name
UniProt Synonym Gene Names
Eno-1; NNE  [Similar Products]
NCBI Summary for NNE
This gene may represent an evolving pseudogene of the alpha-enolase (enolase 1, alpha non-neuron) gene, which has multiple pseudogenes. This gene has an intact open reading frame as well as strong transcriptional support. The length of encoded protein is conserved, compared to the original enolase 1 protein. The exact function of this gene is unknown. [provided by RefSeq, Jul 2008]
UniProt Comments for NNE
ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4 E2|4 81.24 cM

Cellular Component: cell surface; cytoplasm; cytosol; extracellular exosome; extracellular space; extrinsic component of plasma membrane; growth cone; membrane; membrane raft; myelin sheath; neuron projection; nucleus; phosphopyruvate hydratase complex; plasma membrane

Molecular Function: cadherin binding; enzyme binding; GTPase binding; heat shock protein binding; phosphopyruvate hydratase activity; protein heterodimerization activity; protein homodimerization activity; RNA binding

Biological Process: in utero embryonic development; positive regulation of binding
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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