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anti-HCK antibody :: Rabbit anti-Mouse Hemopoietic Cell Kinase Polyclonal Antibody

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Catalog # MBS2026020 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $185 +1 FREE 8GB USB
  0.1 mg  /  $230 +1 FREE 8GB USB
  0.2 mg  /  $345 +1 FREE 8GB USB
  1 mg  /  $825 +1 FREE 8GB USB
Product Name

Hemopoietic Cell Kinase (HCK), Polyclonal Antibody

Full Product Name

Polyclonal Antibody to Hemopoietic Cell Kinase (HCK)

Product Gene Name
Matching Pairs
Matching Pairs
Unconjugated Antibody: Hemopoietic Cell Kinase (MBS2026020)
APC-CY7 Conjugated Antibody: Hemopoietic Cell Kinase (HCK) (MBS2066406)
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for P08103
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against HCK. It has beenselected for its ability to recognize HCK in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
200ug/ml (lot specific)
Fragment
HCK (Gly2~Pro524)
Organism Species
Mus musculus (Mouse)
Conjugate
No Conjugate
Immunogen
Recombinant HCK (Gly2~Pro524) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2066408
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-HCK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-HCK antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-HCK antibody
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.

Western Blot (WB) of anti-HCK antibody
Western Blot: Sample: Recombinant protein.
anti-HCK antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-HCK antibody
DAB staining on fromalin fixed paraffin- embedded Kidney tissue)
anti-HCK antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for HCK. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
56,942 Da
NCBI Official Full Name
tyrosine-protein kinase HCK isoform p56Hck
NCBI Official Synonym Full Names
hemopoietic cell kinase
NCBI Official Symbol
NCBI Official Synonym Symbols
Bmk; AI849071
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NCBI Protein Information
tyrosine-protein kinase HCK
UniProt Protein Name
Tyrosine-protein kinase HCK
UniProt Synonym Protein Names
B-cell/myeloid kinase; BMK; Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK/p59-HCK
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
NCBI Summary for HCK
The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq, Feb 2010]
UniProt Comments for HCK
Hck: a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization.

Protein type: EC 2.7.10.2; Kinase, protein; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Src family; TK group

Chromosomal Location of Human Ortholog: 2 H1|2 75.41 cM

Cellular Component: actin filament; caveola; cell junction; cell projection; cytoplasm; cytoplasmic vesicle; cytoskeleton; extrinsic component of cytoplasmic side of plasma membrane; focal adhesion; Golgi apparatus; lysosome; membrane; nucleoplasm; nucleus; plasma membrane

Molecular Function: ATP binding; kinase activity; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphotyrosine binding; protein binding; protein kinase activity; protein tyrosine kinase activity; receptor binding; transferase activity

Biological Process: cell differentiation; cell migration; defense response to Gram-positive bacterium; exocytosis; immune system process; inflammatory response; innate immune response; negative regulation of apoptosis; peptidyl-tyrosine autophosphorylation; peptidyl-tyrosine phosphorylation; phagocytosis; phosphorylation; positive regulation of actin cytoskeleton reorganization; positive regulation of cell proliferation; protein amino acid phosphorylation; protein autophosphorylation; regulation of cell proliferation; regulation of cell shape; regulation of phagocytosis; regulation of podosome assembly; regulation of transcription factor activity; transmembrane receptor protein tyrosine kinase signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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