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anti-HMG1 antibody :: Rabbit anti-Mouse High Mobility Group Protein 1 (HMG1) Polyclonal Antibody

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Catalog # MBS2007018 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
  0.01 mg  /  $105 +1 FREE 8GB USB
  0.02 mg  /  $125 +1 FREE 8GB USB
  0.05 mg  /  $170 +1 FREE 8GB USB
  0.1 mg  /  $210 +1 FREE 8GB USB
  0.2 mg  /  $315 +1 FREE 8GB USB
  1 mg  /  $745 +1 FREE 8GB USB
Product Name

High Mobility Group Protein 1 (HMG1), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-MGKGDP KKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE
3D Structure
ModBase 3D Structure for P63158
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against HMG1. It has been selected for its ability to recognize HMG1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
200ug/ml (lot specific)
Immunogen
Recombinant HMG1 (Met1~Glu215) expressed in E.coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2041950
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-HMG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-HMG1 antibody
Applications Tested/Suitable for anti-HMG1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes for anti-HMG1 antibody
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200

Western Blot (WB) of anti-HMG1 antibody
Western Blot: Sample: Mouse Kidney lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)
anti-HMG1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-HMG1 antibody
Western Blot: Sample: Mouse Cerebrum lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)
anti-HMG1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-HMG1 antibody
Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)
anti-HMG1 antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-HMG1 antibody
Western Blot: Sample: Recombinant protein.
anti-HMG1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-HMG1 antibody
DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-HMG1 antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-HMG1 antibody
Knockout Validation: Lane 1: Wild-type HepG2 cell lysate;;Lane 2: HMG1 knockout HepG2 cell lysate;;Predicted MW: 25kDa ;Observed MW: 30kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(#MBS2086047)
anti-HMG1 antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for HMG1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
24,894 Da
NCBI Official Full Name
high mobility group protein B1
NCBI Official Synonym Full Names
high mobility group box 1
NCBI Official Symbol
Hmgb1  [Similar Products]
NCBI Official Synonym Symbols
p30; Hmg1; HMG-1; SBP-1
  [Similar Products]
NCBI Protein Information
high mobility group protein B1
UniProt Protein Name
High mobility group protein B1
UniProt Synonym Protein Names
High mobility group protein 1; HMG-1
UniProt Gene Name
Hmgb1  [Similar Products]
UniProt Synonym Gene Names
Hmg-1; Hmg1; HMG-1  [Similar Products]
NCBI Summary for HMG1
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
UniProt Comments for HMG1
HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5 G3|5 89.18 cM

Cellular Component: cytoplasm; early endosome; extracellular region; extracellular space; neuron projection; nucleus

Molecular Function: bent DNA binding; calcium-dependent protein kinase regulator activity; crossed form four-way junction DNA binding; cytokine activity; double-stranded DNA binding; double-stranded RNA binding; open form four-way junction DNA binding; phosphatidylserine binding; protein binding; protein kinase activator activity; single-stranded RNA binding

Biological Process: base-excision repair; chromatin assembly; DNA geometric change; endothelial cell proliferation; eye development; induction of positive chemotaxis; inflammatory response; lung development; macrophage activation involved in immune response; myeloid dendritic cell activation; negative regulation of apoptotic cell clearance; plasmacytoid dendritic cell activation; positive regulation of cell migration; positive regulation of ERK1 and ERK2 cascade; positive regulation of glycogen catabolic process; positive regulation of innate immune response; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of mesenchymal cell proliferation; positive regulation of mitotic cell cycle; positive regulation of myeloid cell differentiation; positive regulation of NIK/NF-kappaB signaling; positive regulation of protein phosphorylation; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; positive regulation of wound healing; regulation of autophagy; regulation of nucleotide-excision repair; regulation of T cell mediated immune response to tumor cell; regulation of tolerance induction; response to glucocorticoid stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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