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anti-Mlkl antibody :: Rabbit anti-Mouse M Mlkl Polyclonal Antibody

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Catalog # MBS9206965
Unit / Price
  0.08 mL  /  $165 +1 FREE 8GB USB
  0.4 mL  /  $370 +1 FREE 8GB USB
Product Name

M Mlkl, Polyclonal Antibody

Also Known As

M Mlkl Antibody (C-term)

Product Synonym Names
Mixed lineage kinase domain-like protein; Mlkl {ECO:0000312|EMBL:AAH237551; ECO:0000312|MGI:MGI:1921818}
Antibody/Peptide Pairs
M Mlkl peptide (MBS9220629) is used for blocking the activity of M Mlkl antibody (MBS9206965)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Positions
444-472
Immunogen Sequence Length
464
3D Structure
ModBase 3D Structure for Q9D2Y4
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Mouse
Specificity
This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (lot specific)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Other Notes
Small volumes of anti-Mlkl antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Mlkl antibody
The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.
Applications Tested/Suitable for anti-Mlkl antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-Mlkl antibody
WB~~1:1000

Western Blot (WB) of anti-Mlkl antibody
All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution
Lane 1: mouse testis lysates
Lane 2: mouse liver lysates
Lane 3: NIH/3T3 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-Mlkl antibody
All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution
Lane 1: NIH/3T3 whole cell lysates
Lane 2: mouse spleen lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-Mlkl antibody
Anti-Mlkl Antibody (C-term)at 1:1000 dilution + mouse lung lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-Mlkl antibody
Anti-Mlkl Antibody (C-term)at 1:2000 dilution + NIH/3T3 whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-Mlkl antibody
All lanes : Anti-Mlkl Antibody (C-term) at 1:1000 dilution
Lane 1: mouse lung lysates
Lane 2: mouse spleen lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-Mlkl antibody
All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution
Lane 1: mouse lung lysates
Lane 2: NIH/3T3 whole cell lysates
Lane 3: mouse liver lysates
Lane 4: mouse testis whole cell lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 54 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
anti-Mlkl antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-Mlkl antibody
Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term). MBS9206965 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
anti-Mlkl antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for Mlkl. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
54317
NCBI Official Full Name
mixed lineage kinase domain-like protein
NCBI Official Synonym Full Names
mixed lineage kinase domain-like
NCBI Official Symbol
NCBI Official Synonym Symbols
9130019I15Rik
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NCBI Protein Information
mixed lineage kinase domain-like protein
UniProt Protein Name
Mixed lineage kinase domain-like protein
UniProt Gene Name
UniProt Entry Name
MLKL_MOUSE
NCBI Summary for Mlkl
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Comments for Mlkl
MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; TKL-Unique family

Cellular Component: membrane; cytoplasm; plasma membrane

Molecular Function: transferase activity, transferring phosphorus-containing groups; nucleotide binding; protein complex binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: programmed cell death; protein amino acid phosphorylation
Product References and Citations for anti-Mlkl antibody
Bisson, N., et al. Cell Cycle 7(7):909-916(2008)

Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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