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anti-Ifih1 antibody :: Goat MDA5/Ifih1 Polyclonal Antibody

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Catalog # MBS420601
Unit / Price
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  0.1 mg  /  $225 +1 FREE 8GB USB
anti-Ifih1 antibody
Product Name

MDA5/Ifih1, Polyclonal Antibody

Popular Item
Full Product Name

Goat anti-MDA5/Ifih1 (mouse) Antibody

Product Synonym Names
Ifih1; interferon induced with helicase C domain 1; 9130009C22Rik; Helicard; Hlcd; MDA-5; MDA5; MGC90959; melanoma differentiation associated protein-5; Ifih1 antibody; interferon induced with helicase C domain 1 antibody; 9130009C22Rik antibody; Helicard antibody; Hlcd antibody; MDA-5 antibody; MDA5 antibody; MGC90959 antibody; melanoma differentiation associated protein-5 antibody; MDA5 / Ifih1 (mouse)
Product Synonym Gene Name
Antibody/Peptide Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence
KQKLRNNDLK KQN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Mouse
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-KQKLRNNDLKKQN, from the internal region of the protein sequence according to NP_082111.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes
Small volumes of anti-Ifih1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Ifih1 antibody
Peptide ELISA (EIA)
Application Notes for anti-Ifih1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave an approx 80kDa band in Mouse Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_082111.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.
NCBI/Uniprot data below describe general gene information for Ifih1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
110,842 Da
NCBI Official Full Name
interferon induced with helicase C domain 1
NCBI Official Synonym Full Names
interferon induced with helicase C domain 1
NCBI Official Symbol
Ifih1  [Similar Products]
NCBI Official Synonym Symbols
Hlcd; MDA5; RLR-2; Helicard; 9130009C22Rik
  [Similar Products]
NCBI Protein Information
interferon-induced helicase C domain-containing protein 1
UniProt Protein Name
Interferon-induced helicase C domain-containing protein 1
UniProt Synonym Protein Names
Helicase with 2 CARD domains; Helicard; Interferon induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; RIG-I-like receptor 2; RLR-2
UniProt Gene Name
Ifih1  [Similar Products]
UniProt Synonym Gene Names
Helicard; MDA-5; RLR-2  [Similar Products]
UniProt Entry Name
IFIH1_MOUSE
UniProt Comments for Ifih1
IFIH1: Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O- methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV) and mengo encephalomyocarditis virus (ENMG). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines. Monomer in the absence of ligands and homodimerizes in the presence of dsRNA ligands. Can assemble into helical or linear polymeric filaments on long dsRNA. Interacts with MAVS/IPS1. Interacts with V protein of Simian virus 5, Human parainfluenza virus 2, Mumps virus, Sendai virus and Hendra virus. Binding to paramyxoviruses V proteins prevents IFN-beta induction, and the further establishment of an antiviral state. Interacts with PCBP2. Interacts with NLRC5. Interacts with PIAS2-beta. Interacts with DDX60. By interferon (IFN) and TNF. Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung. Belongs to the helicase family. RLR subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.4.13; Apoptosis; Helicase

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; DNA binding; double-stranded RNA binding; helicase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides; metal ion binding; nucleotide binding; protein binding; ribonucleoprotein binding; RNA binding; single-stranded RNA binding; zinc ion binding

Biological Process: defense response to virus; immune system process; innate immune response; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; protein sumoylation; response to virus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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