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anti-Mepe antibody :: Sheep anti-Mouse MEPE, ED Polyclonal Antibody

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Catalog # MBS627449
Unit / Price
  0.1 mg  /  $765 +1 FREE 8GB USB
anti-Mepe antibody
Product Name

MEPE, ED, Polyclonal Antibody

Popular Item
Also Known As

MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45, OF45)

Product Synonym Names
Anti -MEPE, ED (Matrix Extracellular Phosphoglycoprotein, Osteoblast/Osteocyte Factor 45, OF45)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 5; NC_000071.6 (104325329..104338611). Location: 5 E4; 5
Clonality
Polyclonal
Isotype
IgG
Host
Sheep
Species Reactivity
Mouse
Specificity
Recognizes the extracellular domain of mouse MEPE.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Concentration
~0.2 mg/ml (after reconstitution) (lot specific)
Immunogen
Recombinant protein corresponding to aa17-433 at the etracellular domain of mouse MEPE ezpressed in NSO cells (ak70342). Species Sequence Homomlogy: Rat; 77%, human; 57%
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Other Notes
Small volumes of anti-Mepe antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-Mepe antibody
MEPE (matrix extracellular phosphoglycoprotein; also OF45) is a 56-58kD member of the SIBLING protein family. It is expressed by osteocytes, osteoblasts, odontoblasts and chondrocytes. MEPE is found both intra- and extracellularly. Within the cell, it binds to CHK1. This protects CHK1 from degradation, and the cell from DNA damage. Extracellularly, it binds to Phex, which protects it from cathepsin B proteolysis. Undegraded, it promotes bone vascularization and blocks bone resorption. Following cleavage after Arg410 (in mouse), a 2kD ASARM fragment is generated that, when phosphorylated, blocks mineralization. Mature mouse MEPE is 417aa in length. It contains an RGD motif and ASARM domain (aa416-433). In addition to its 57kD form, 70kD, 42-45kD and 30-35kD forms of mouse MEPE have been reported. Whether they are cleavage forms or products of glycosylation is unclear. Over aa147-433, mouse MEPE shares 77% and 57% aa identity with rat and human MEPE, respectively.
Applications Tested/Suitable for anti-Mepe antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes for anti-Mepe antibody
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml.
ELISA (direct): 0.5-1ug/ml
NCBI/Uniprot data below describe general gene information for Mepe. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
matrix extracellular phosphoglycoprotein with ASARM motif
NCBI Official Synonym Full Names
matrix extracellular phosphoglycoprotein with ASARM motif (bone)
NCBI Official Symbol
NCBI Official Synonym Symbols
NCBI Protein Information
matrix extracellular phosphoglycoprotein with ASARM motif; osteoregulin
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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