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anti-MARS antibody :: Rabbit anti-Mouse Methionyl tRNA Synthetase Polyclonal Antibody

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Catalog # MBS2034027 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
Scan QR to view Datasheet
  0.01 mg  /  $110 +1 FREE 8GB USB
  0.02 mg  /  $130 +1 FREE 8GB USB
  0.05 mg  /  $185 +1 FREE 8GB USB
  0.1 mg  /  $230 +1 FREE 8GB USB
  0.2 mg  /  $345 +1 FREE 8GB USB
  1 mg  /  $825 +1 FREE 8GB USB
Product Name

Methionyl tRNA Synthetase (MARS), Polyclonal Antibody

Popular Item
Full Product Name

Polyclonal Antibody to Methionyl tRNA Synthetase (MARS)

Product Synonym Names
METRS; MTRNS; MetRS; Methionine tRNA Ligase 1,Cytoplasmic
Matching Pairs
Matching Pairs
Matching Pairs
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
3D Structure
ModBase 3D Structure for Q68FL6
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Mouse
Specificity
The antibody is a rabbit polyclonal antibody raised against MARS. It has been selected for its ability to recognize MARS in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, Containing 0.02% NaN3, 50% glycerol.
Concentration
200ug/ml (lot specific)
Fragment
MARS (Gly74~Pro212)
Conjugate
No Conjugate
Immunoglobulin Type
IgG
Quality Control
Content: The quality control contains recombinant MARS (Gly74~Pro212) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2069428
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Other Notes
Small volumes of anti-MARS antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MARS antibody
Applications Tested/Suitable for anti-MARS antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes for anti-MARS antibody
Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000

Western Blot (WB) of anti-MARS antibody
Western Blot: Sample: Human HepG2 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse MARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-MARS antibody Western Blot (WB) (WB) image
Western Blot (WB) of anti-MARS antibody
Western Blot: Sample: Recombinant protein.
anti-MARS antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-MARS antibody
DAB staining on fromalin fixed paraffin- embedded tissue)
anti-MARS antibody Immunohistochemistry (IHC) (IHC) image
Immunohistochemistry (IHC) of anti-MARS antibody
DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
anti-MARS antibody Immunohistochemistry (IHC) (IHC) image
Knockout Validation of anti-MARS antibody
Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: MARS knockout HepG2 cell lysate; Predicted MW: 101kd Observed MW: 90kd Primary Ab: 1ug/ml Rabbit Anti-Mouse MARS Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)
anti-MARS antibody Knockout Validation image
NCBI/Uniprot data below describe general gene information for MARS. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Related Accession #
Molecular Weight
101,431 Da
NCBI Official Full Name
methionine--tRNA ligase, cytoplasmic isoform 2
NCBI Official Synonym Full Names
methionine-tRNA synthetase
NCBI Official Symbol
NCBI Official Synonym Symbols
Metrs; Mtrns
  [Similar Products]
NCBI Protein Information
methionine--tRNA ligase, cytoplasmic
UniProt Protein Name
Methionine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names
Methionyl-tRNA synthetase; MetRS
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
MetRS  [Similar Products]
NCBI Summary for MARS
The encoded protein belongs to the class I family of tRNA synthetases, a class of enzymes that charge tRNAs with their cognate amino acids. The related human gene product is essential for the translation initiation of mRNAs. This gene has an overlapping 3' UTR tail-to-tail arrangement with an adjacent gene on the opposite strand that encodes an inhibitor of the CCAAT/enhancer-binding protein's DNA binding activity. This arrangement, conserved in human and mouse, may be involved in mRNA stability and possible functional and regulatory interaction of these adjacent overlapping genes. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2010]
UniProt Comments for MARS
MARS: a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]

Protein type: Aminoacyl-tRNA synthetase; EC 6.1.1.10; Ligase; Other Amino Acids Metabolism - selenoamino acid; Translation; Translation regulation

Chromosomal Location of Human Ortholog: 10|10 D3

Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cytoplasm; cytosol; membrane; mitochondrion; nucleolus

Molecular Function: aminoacyl-tRNA ligase activity; ATP binding; ligase activity; methionine-tRNA ligase activity; nucleotide binding; RNA binding; tRNA binding

Biological Process: cellular response to insulin stimulus; cellular response to starvation; methionyl-tRNA aminoacylation; rRNA transcription; translation; tRNA aminoacylation for protein translation
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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