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anti-MIF antibody :: anti-Mouse MIF Polyclonal Antibody

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Catalog # MBS551195
Unit / Price
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  0.05 mg  /  $260 +1 FREE 8GB USB
  0.2 mg  /  $610 +1 FREE 8GB USB
  1 mg  /  $2,145 +2 FREE 8GB USB
anti-MIF antibody
Product Name

MIF, Polyclonal Antibody

Popular Item
Full Product Name

Mouse MIF Affinity Purified Polyclonal

Product Synonym Names
RAA25032; polyclonal; MIF
Product Gene Name
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Chromosome Location
Chromosome: 22; NC_000022.10 (24236565..24237409). Location: 22q11.23
OMIM
153620
3D Structure
ModBase 3D Structure for P14174
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Polyclonal anti MIF was produced for sera of rabbits immunized with recombinal Mouse MIF.
Species Reactivity
Mouse
Purity/Purification
Purification: Serum IgG fraction was purified by affinity chromatography using immobilized recombinant mouse MIF.
Purity: >95%
Form/Format
Sterile filtered PBS pH 7.4 solution and %5 trehalose.
Concentration
0.2mg/ml (lot specific)
Host Note
Polyclonal Mouse MIF antibody was produced from sera of rabbits immunized with recombinant Mouse MIF
Presentation
Sterile solution from 0.2um filtered solution in PBS pH 7.4.
Dilution
A quick spin of the vial followed by reconstitution in PBS pH 7.4 or other diluents.
Preparation and Storage
This antibody can be stored in working aliquots at 2 - 8 degree C for one month, or at -20 degree for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Other Notes
Small volumes of anti-MIF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Product Categories/Family for anti-MIF antibody
Applications Tested/Suitable for anti-MIF antibody
Western Blot (WB), ELISA
Application Notes for anti-MIF antibody
Western Blot (WB): Used at a concentration range of 2-10 ug/ml, with proper secondary antibody.
ELISA: Antibody detection limit is 0.04ng/well using recombinant Mouse MIF (MBS553301). Use at a concentration range of 0.1-0.2 ug/mL with proper secondary antibody.
NCBI/Uniprot data below describe general gene information for MIF. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,476 Da
NCBI Official Full Name
MIF
NCBI Official Synonym Full Names
macrophage migration inhibitory factor (glycosylation-inhibiting factor)
NCBI Official Symbol
NCBI Official Synonym Symbols
GIF; GLIF; MMIF
  [Similar Products]
NCBI Protein Information
macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase
UniProt Protein Name
Macrophage migration inhibitory factor
UniProt Synonym Protein Names
Glycosylation-inhibiting factor; GIF; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase
Protein Family
UniProt Gene Name
UniProt Synonym Gene Names
GLIF; MMIF; MIF; GIF  [Similar Products]
UniProt Entry Name
MIF_HUMAN
NCBI Summary for MIF
This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq, Jul 2008]
UniProt Comments for MIF
MIF: a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. Also acts as a phenylpyruvate tautomerase.

Protein type: EC 5.3.2.1; Apoptosis; Cytokine; EC 5.3.3.12; Cell development/differentiation; Isomerase; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: nucleoplasm; extracellular space; cell surface; cytoplasm; extracellular region; vesicle

Molecular Function: phenylpyruvate tautomerase activity; protein binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; dopachrome isomerase activity; receptor binding; chemoattractant activity

Biological Process: DNA damage response, signal transduction by p53 class mediator; regulation of macrophage activation; positive regulation of lipopolysaccharide-mediated signaling pathway; carboxylic acid metabolic process; cell aging; negative regulation of cellular protein metabolic process; prostaglandin biosynthetic process; positive regulation of peptidyl-serine phosphorylation; cell proliferation; positive regulation of MAP kinase activity; negative regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of fibroblast proliferation; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of myeloid cell apoptosis; cell surface receptor linked signal transduction; negative regulation of mature B cell apoptosis; positive chemotaxis; positive regulation of B cell proliferation; innate immune response; inflammatory response; positive regulation of phosphorylation; negative regulation of apoptosis; positive regulation of cytokine secretion

Disease: Rheumatoid Arthritis, Systemic Juvenile
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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