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anti-Cdkn2a antibody :: Goat P19ARF Polyclonal Antibody

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Catalog # MBS422702
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

P19ARF (Cdkn2a), Polyclonal Antibody

Also Known As

Goat anti-P19ARF (mouse) Antibody

Product Synonym Names
Cdkn2a; cyclin-dependent kinase inhibitor 2A; RP23-166I8.5; ARF-INK4a; Arf; INK4a-ARF; Ink4a/Arf; MTS1; Pctr1; p16; p16(INK4a); p16INK4a; p19ARF; CDK4I; OTTMUSP00000008143; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16,; Arf antibody; ARF-INK4a antibody; CDK4I antibody; cyclin-dependent kinase 4 inhibitor A antibody; cyclin-dependent kinase inhibitor 2A antibody; cyclin-dependent kinase inhibitor 2A (p16; inhibits CDK4) antibody; cyclin-dependent kinase inhibitor 2A; isoforms 1/2 antibody; cyclin-dependent kinase inhibitor protein antibody; Ink4a/Arf antibody; INK4a-ARF antibody; mitochondrial smARF antibody; MTS1 antibody; OTTMUSP00000008143 antibody; p16 antibody; p16(INK4a) antibody; p16-INK4 antibody; p16INK4a antibody; p16-INK4a antibody; p19ARF antibody; Pctr1 antibody; RP23-166I8.5 antibody; Cdkn2a antibody; P19ARF (mouse)
Product Synonym Gene Name
Antibody/Peptide Pairs
P19ARF peptide (MBS427702) is used for blocking the activity of P19ARF antibody (MBS422702)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
169
Immunogen Sequence
RRGPHRNPGP GDDD
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Mouse; Expected from sequence similarity: Mouse
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-RRGPHRNPGPGDDD, from the internal region of the protein sequence according to NP_034007.1.
Epitope
Internal region
Note
This antibody is expected to recognize isoform p19ARF (NP_034007.1) only.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8613
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-Cdkn2a antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-Cdkn2a antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-Cdkn2a antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 18kDa band observed in Mouse Kidney and Liver lysates (calculated MW of 19.2kDa according to NP_034007.1). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-Cdkn2a antibody
(0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-Cdkn2a antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for Cdkn2a. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
17,941 Da
NCBI Official Full Name
cyclin-dependent kinase inhibitor 2A isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol
Cdkn2a  [Similar Products]
NCBI Official Synonym Symbols
Arf; p16; MTS1; Pctr1; p19ARF; p16INK4a; p19<ARF>; ARF-INK4a; INK4a-ARF; Ink4a/Arf; p16(INK4a)
  [Similar Products]
NCBI Protein Information
cyclin-dependent kinase inhibitor 2A
UniProt Protein Name
Tumor suppressor ARF
UniProt Synonym Protein Names
Alternative reading frame; ARF; Cyclin-dependent kinase inhibitor 2A; p19ARF
Protein Family
UniProt Gene Name
Cdkn2a  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
ARF_MOUSE
UniProt Comments for Cdkn2a
p16-INK4A iso3: a cell-cycle regulatory protein that interacts with CDK4 and CDK6, inhibiting their ability to interact with cyclins D. Inhibits the phosphorylation of the retinoblastoma protein by CDK4 or CDK6, and entry into the S phase of the cell cycle. The p16INK4A and p14ARF proteins are encoded by CDKN2A, a known tumour suppressor gene in multiple cancers. CDKN2A is inactivated in 72% of cases of lung squamous cell carcinoma: 21% by epigenetic silencing by methylation, 18% inactivating mutation, 4% by exon 1b skipping, and 29% by homozygous deletion. Defects in CDKN2A are the cause of familial atypical multiple mole melanoma-pancreatic carcinoma syndrome, Li-Fraumeni syndrome, and the melanoma-astrocytoma syndrome. The melanoma-astrocytoma syndrome is characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma. Four alternatively spliced p16 isoforms have been reported. Two alternatively spliced isoforms of ARF have been reported.

Protein type: Tumor suppressor; Cell cycle regulation; Nucleolus

Cellular Component: cytoplasm; granular component; mitochondrion; nuclear body; nucleolus; nucleoplasm; nucleus; protein complex

Molecular Function: cyclin-dependent protein kinase inhibitor activity; DNA binding; NF-kappaB binding; p53 binding; protein binding; protein kinase binding; protein N-terminus binding; SUMO ligase activity; transcription factor activity; transcription factor binding; ubiquitin-protein ligase inhibitor activity

Biological Process: aging; apoptosis; apoptotic mitochondrial changes; caspase activation; cell aging; cell cycle; cell cycle arrest; epidermis development; G1/S transition of mitotic cell cycle; glucose homeostasis; inhibition of NF-kappaB transcription factor; mitochondrial depolarization; mitochondrion degradation; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of cell-matrix adhesion; negative regulation of cyclin-dependent protein kinase activity; negative regulation of immature T cell proliferation in the thymus; negative regulation of mammary gland epithelial cell proliferation; negative regulation of phosphorylation; negative regulation of protein binding; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; negative regulation of ubiquitin-protein ligase activity; positive regulation of apoptosis; positive regulation of apoptosis involved in mammary gland involution; positive regulation of DNA damage response, signal transduction by p53 class mediator; positive regulation of protein sumoylation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein destabilization; protein polyubiquitination; protein stabilization; regulation of cyclin-dependent protein kinase activity; regulation of G2/M transition of mitotic cell cycle; regulation of gene expression; regulation of nucleocytoplasmic transport; regulation of protein export from nucleus; regulation of protein stability; regulation of transcription, DNA-dependent; response to drug; response to organic cyclic substance; response to organic nitrogen; rRNA processing; rRNA transcription; somatic stem cell division; somatic stem cell maintenance; transcription, DNA-dependent
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

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