• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

anti-4E-BP1 antibody :: Rabbit anti-Mouse, Rat 4E-BP1 Polyclonal Antibody

Scan QR to view Datasheet
Catalog # MBS9402298
Unit / Price
Scan QR to view Datasheet
  0.05 mL  /  $200 +1 FREE 8GB USB
  0.1 mL  /  $255 +1 FREE 8GB USB
Product Name

4E-BP1, Polyclonal Antibody

Full Product Name

4E-BP1 (Ab-69) Antibody

Product Synonym Names
4EBP1; EIF4EBP1; P/OKCL.6; PHAS-1
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602223
3D Structure
ModBase 3D Structure for Q13541
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, Rat
Specificity
The antibody detects endogenous levels of total 4E-BP1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (lot specific)
Immunogen Type
Peptide
Immunogen Description
Synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 70 (A-K-T-P-P).
Target Name
4E-BP1
Preparation and Storage
Store at -20 degree C
Other Notes
Small volumes of anti-4E-BP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-4E-BP1 antibody
Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.Anne-Claude Gingras, Genes & Dev., Jun 1999; 13: 1422. Guangming Liu, J. Biol. Chem., Mar 2002; 277: 8810 - 8816. Ya-Ping Jiang, J. Biol. Chem., Mar 2001; 276: 10943 - 10951. Anne-Claude Gingras, Genes & Dev., Feb 1998; 12: 502.
Product Categories/Family for anti-4E-BP1 antibody
Applications Tested/Suitable for anti-4E-BP1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-4E-BP1 antibody
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Western Blot (WB) of anti-4E-BP1 antibody
Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 30mins), using 4E-BP1 (Ab-69) antibody.
anti-4E-BP1 antibody Western Blot (WB) (WB) image
Immunohistochemistry (IHC) of anti-4E-BP1 antibody
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Ab-69) antibody.
anti-4E-BP1 antibody Immunohistochemistry (IHC) (IHC) image
NCBI/Uniprot data below describe general gene information for 4E-BP1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
12,580 Da
NCBI Official Full Name
eukaryotic translation initiation factor 4E-binding protein 1
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E binding protein 1
NCBI Official Symbol
EIF4EBP1  [Similar Products]
NCBI Official Synonym Symbols
BP-1; 4EBP1; 4E-BP1; PHAS-I
  [Similar Products]
NCBI Protein Information
eukaryotic translation initiation factor 4E-binding protein 1
UniProt Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
UniProt Synonym Protein Names
Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
UniProt Gene Name
EIF4EBP1  [Similar Products]
UniProt Synonym Gene Names
4E-BP1; eIF4E-binding protein 1; PHAS-I  [Similar Products]
UniProt Entry Name
4EBP1_HUMAN
NCBI Summary for 4E-BP1
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
UniProt Comments for 4E-BP1
4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; TOR signaling pathway; cellular protein metabolic process; response to ethanol; translation; insulin receptor signaling pathway; translational initiation; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000